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| Status |
Public on Jun 14, 2016 |
| Title |
Analysis of changes in gene expression in skin epidermis upon conditional knockout of PRC2-subunit Eed |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of changes in gene expression in skin epidermis upon conditional knockout of the essential Polycomb repressive complex 2 (PRC2) subunit Eed. Loss of Eed in skin epithelium leads to de-repression of key Merkel-differentiation genes, which are known PRC2 targets, and results in ectopic formation of Merkel cells that are associated with all hair types.
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| Overall design |
Gene expression analysis: To determine the changes in gene expression in skin epidermis upon conditional knockout of Eed, total RNA was isolated from skin epidermis in four biologic replicates from cells in different conditions and hybridized to SurePrint G3 Mouse GE 8X60K microarrays (Agilent).
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| Contributor(s) |
Perdigoto CN, Dauber KL, Cohen I, Ezhkova E |
| Citation(s) |
27414999 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR063724 |
Role of Chromatin Regulators in Skin Control |
MOUNT SINAI SCHOOL OF MEDICINE |
Ezhkova |
| R01 AR069078 |
Toward Understanding the Role of the Polycomb Complex in Skin Control |
MOUNT SINAI SCHOOL OF MEDICINE |
Ezhkova |
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| |
| Submission date |
Jun 11, 2016 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Idan Cohen |
| E-mail(s) |
idancoh29@gmail.com
|
| Phone |
+972-58-4266754
|
| Organization name |
Ben-Gurion University
|
| Department |
Microbiology, Immunology and Genetics
|
| Lab |
Idan Cohen
|
| Street address |
David Ben Gurion Blvd 1
|
| City |
Beer Sheva |
| ZIP/Postal code |
84105 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (8)
|
| GSM2197565 |
Eed conditional KO skin epidermis replicate 1 |
| GSM2197566 |
Eed conditional KO skin epidermis replicate 2 |
| GSM2197567 |
Eed conditional KO skin epidermis replicate 3 |
| GSM2197568 |
Eed conditional KO skin epidermis replicate 4 |
| GSM2197569 |
Control newborn skin epidermis replicate 1 |
| GSM2197570 |
Control newborn skin epidermis replicate 2 |
| GSM2197571 |
Control newborn skin epidermis replicate 3 |
| GSM2197572 |
Control newborn skin epidermis replicate 4 |
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| Relations |
| BioProject |
PRJNA325391 |
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