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| Status |
Public on Nov 22, 2016 |
| Title |
Trichloroethylene-induced miRNA expression changes in B6C3F1 mouse liver |
| Platform organism |
synthetic construct |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To know if Trichloroethylene (TCE) can induce miRNA expression changes in mouse liver, we use microarray to screen for differentially expressed miRNAs in the liver of mice exposed to TCE at a dose of 1000 mg/kg b.w. for 5days . In our results, nine miRNAs were selected by volcano plot filtering (fold change ≥ 2 and P-value ≤ 0.05), out of which eight were up-regulated and one was down-regulated. The differential expression of miR-182-5p, miR-34a-5p and miR-451a were validated by qPCR.
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| Overall design |
Six B6C3F1 male mice were oral administrated with either corn oil or TCE (dissolved in corn oil, 1000mg/kg b.w per day) for 5 days. The expression changes of miRNAs in TCE exposured mouse liver were screened by microarray and were validated by qPCR.
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| Contributor(s) |
Chen T, Jiang Y |
| Citation(s) |
28013219 |
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| Submission date |
Aug 22, 2016 |
| Last update date |
Jul 27, 2018 |
| Contact name |
Tao Chen |
| E-mail(s) |
tchen@suda.edu.cn
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| Phone |
8651265882273
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| Organization name |
Soochow University
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| Street address |
199 Ren-Ai-rd
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| City |
SuZhou |
| State/province |
JiangSu |
| ZIP/Postal code |
215123 |
| Country |
China |
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| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA339729 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85904_RAW.tar |
26.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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