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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 04, 2007 |
| Title |
The microRNAs miR-373 and miR-520c promote tumor migration, invasion and metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNAs (miRNAs) are single-stranded noncoding RNAs that play important roles in many biological processes. Although the oncogenic and tumor suppressive functions of several miRNAs have been characterized, the role of miRNAs in mediating tumor metastasis was only recently addressed and still remain largely unexplored. To identify potential metastasis-promoting miRNAs, we set up a genetic screen using a non-metastatic human breast tumor cell line that was transduced with a miRNA expression library and subjected to a trans-well migration assay. We found human miR-373 and 520c to stimulate cancer cell migration as well as tumor cell invasion in vitro and in vivo, and that certain cancer cell lines depend on endogenous miR-373 activity to migrate efficiently. Mechanistically, the migration phenotype of miR-373 and miR-520c can be explained by their suppression of CD44 expression. Finally, we found significant up-regulation of miR-373 expression in clinical breast cancer primary and metastasis samples that inversely correlated with CD44 expression. Taken together, our study indicates that miRNAs are involved in tumor migration and invasion and implicates miR-373 and conceivably miR-520c as metastasis-promoting miRNAs.
Keywords: human breast cancer cell MCF7 vs MCF7 expressing miR-373 vs MCF7 expressing miR-520c
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| Overall design |
Illumina human v6 array was used for MCF7 cells, MCF7 cells stably expressing miR-373 and MCF7 cells stably expressing miR-520c. Triplicate samples were used to performe gene expression analysis on each cell lines.
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| Contributor(s) |
Huang Q, Gumireddy K, Schrier M, le Sage C, Nagel R, Nair S, Egan DA, Li A, Huang G, Klein-Szanto AJ, Gimotty PA, Katsaros D, Coukos G, Zhang L, Pure E, Agami R |
| Citation(s) |
18193036 |
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| Submission date |
Nov 30, 2007 |
| Last update date |
Sep 19, 2012 |
| Contact name |
Louise C Showe |
| E-mail(s) |
lshowe@wistar.org
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| Phone |
215-898-3791
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| Organization name |
The Wistar Institute
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| Lab |
Dr. Louise Showe
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| Street address |
3601 Spruce St
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL6171 |
Wistar Illumina Human V6 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA103659 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9742_RAW.tar |
4.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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