|
| Status |
Public on Nov 06, 2012 |
| Title |
cdc55_24_Msn2DNES_20min |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
reference: untreated W303 msn2msn4
|
| Organism |
Saccharomyces cerevisiae W303 |
| Characteristics |
cell type: whole cell extract
genotype/variation: msn2msn4 pMsn2pMsn2DNES
treatment: none
|
| Treatment protocol |
Hyperosmotic stress: NaCl was added to a final concentration of 0.4M.
|
| Growth protocol |
Cells were grown for 4 generations in 50ml cultures in YPD at 30°C to OD600nm of about 1. before NaCl was added to a final concentration of 0.4M. After 0(untreated control), 10, 20 and 30 minutes cells were harvested and immediately frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using glass beads and phenol/chloroform extraction.
|
| Label |
Cy3
|
| Label protocol |
1 µg of total RNA was used for labeling reaction (Agilent Quick Amp Labeling Kit, two-color, Cat.Nr. 5190-0444). The standard Agilent Protocol for two-color labeling was used, Publication Number: G4140-90050 v.6.0). W303msn2msn4 untreated is Cy3 labelled reference
|
| |
|
| Channel 2 |
| Source name |
Saccharomyces cerevisiae strain W303 msn2msn4cdc55
|
| Organism |
Saccharomyces cerevisiae W303 |
| Characteristics |
cell type: whole cell extract
genotype/variation: msn2msn4cdc55 pMsn2pMsn2DNES
treatment: 0.4M NaCl
|
| Treatment protocol |
Hyperosmotic stress: NaCl was added to a final concentration of 0.4M.
|
| Growth protocol |
Cells were grown for 4 generations in 50ml cultures in YPD at 30°C to OD600nm of about 1. before NaCl was added to a final concentration of 0.4M. After 0(untreated control), 10, 20 and 30 minutes cells were harvested and immediately frozen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using glass beads and phenol/chloroform extraction.
|
| Label |
Cy5
|
| Label protocol |
1 µg of total RNA was used for labeling reaction (Agilent Quick Amp Labeling Kit, two-color, Cat.Nr. 5190-0444). The standard Agilent Protocol for two-color labeling was used, Publication Number: G4140-90050 v.6.0). W303msn2msn4 untreated is Cy3 labelled reference
|
| |
|
| |
| Hybridization protocol |
325ng of both Cy3 and Cy5 labeled cRNA were hybridized to the S. cerevisiae AMADID GE 8x15K G4813A arrays. Samples were hybridized for 17 hours in G2545A Hybridization Oven.
|
| Scan protocol |
Agilent G2565A Microarray Scanner System was used to scan the arrays.
|
| Data processing |
Agilent Feature Extraction program (Version FE 10.5.1.1). Features with signals below 100 units were discarded. Technical replicates were averaged.
|
| |
|
| Submission date |
Nov 05, 2012 |
| Last update date |
Nov 06, 2012 |
| Contact name |
Christoph Schueller |
| E-mail(s) |
Christoph.schueller@boku.ac.at
|
| Organization name |
University of Natural Resources and Life Sciences, Vienna (BOKU)
|
| Department |
Department of Applied Genetics and Cell Biology
|
| Lab |
Schuller
|
| Street address |
Konrad Lorenz Strasse 24
|
| City |
Tulln |
| ZIP/Postal code |
3430 |
| Country |
Austria |
| |
|
| Platform ID |
GPL16244 |
| Series (2) |
| GSE42033 |
The yeast PP2A-CDC55 phosphatase regulates the transcriptional response to hyperosmolarity stress by regulating Msn2 and Msn4 [Time course 2] |
| GSE42034 |
The yeast PP2A-CDC55 phosphatase regulates the transcriptional response to hyperosmolarity stress by regulating Msn2 and Msn4 |
|
