|
| Status |
Public on Nov 22, 2012 |
| Title |
cDNA from wild type cells rep1 |
| Sample type |
RNA |
| |
|
| Source name |
wild type cells
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: K699
genotype/variation: MATa
|
| Treatment protocol |
Exponentially growing cells were harvested.
|
| Growth protocol |
Cells were grown in YP + 2% Glucose to an OD600 of 0.3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted following the manufacturers instructions (QUIAGEN). dscDNA was amplified using IVT (Invitrogen).
|
| Label |
Biotin-N6-ddATP
|
| Label protocol |
10 mg of dscDNA was digested with DNase I and end-labeled using biotin-N6-ddATP using terminal transferase TdT (Roche, 400 U/µl) and Biotin-N6-ddATP (Enzo Life Sciences).
|
| |
|
| Hybridization protocol |
Hybridization was performed following the manufacturers instructions (Affymetrix). Samples were hybridized in buffer containing SSPE, TritonX-100, denatured salmon sperm DNA and biotin-labelled control oligonucleotide, oligo B2. Samples were denatured at 95ºC for 10 minutes before being hybridized to the arrays for 16 hours at 42ºC in a GeneChip Hybridization Oven 640.
|
| Scan protocol |
The washing and scanning protocol (FlexMini_euk2v3_450), provided by Affymetrix, was performed automatically on a GeneChip Fluidics Station 450. Arrays were scanned using the GeneChip Scanner 3000 7G, and primary analysis of the chip data was performed using the Affymetrix GeneChip Operating Software (GCOS).
|
| Description |
K699_29052012
51130800921268011914415882843535.CEL
|
| Data processing |
The data provided was pre-processed and normalised using the standard RMA procedure, as provided by the affy package in Bioconductor 2.11.
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Nov 22, 2012 |
| Contact name |
Rahul Thadani |
| E-mail(s) |
rahul.thadani@cancer.org.uk
|
| Organization name |
Cancer Research UK
|
| Lab |
Chromosome Segregation
|
| Street address |
44 Lincoln's Inn Fields
|
| City |
London |
| ZIP/Postal code |
WC2A 3LY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL2529 |
| Series (2) |
| GSE42458 |
Budding yeast Wapl controls sister chromatid cohesion maintenance and the chromosome condensation status [expression] |
| GSE42460 |
Budding yeast Wapl controls sister chromatid cohesion maintenance and the chromosome condensation status |
|
