|
| Status |
Public on Dec 29, 2014 |
| Title |
Neu_2297 |
| Sample type |
RNA |
| |
|
| Source name |
breast cancer
|
| Organism |
Mus musculus |
| Characteristics |
tissue: breast cancer
strain: FVB
genotype/variation: Neu
|
| Treatment protocol |
Tumors were extracted at endpoint and were flash frozen.
|
| Growth protocol |
MMTV-Neu tumors were induced in the indicated E2F wildtype or knockout background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy was used to extract RNA following manufacturer guidelines.
|
| Label |
biotin
|
| Label protocol |
standard affymetrix protocol
|
| |
|
| Hybridization protocol |
standard affymetrix protocol
|
| Scan protocol |
standard affymetrix protocol
|
| Description |
Neu_2297
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. Normalization was with Affymetrix Expression Console using build 1.2.1.20.
|
| |
|
| Submission date |
Nov 27, 2012 |
| Last update date |
Dec 29, 2014 |
| Contact name |
Eran Andrechek |
| Phone |
517-884-5042
|
| Organization name |
Michigan State University
|
| Department |
Physiology
|
| Lab |
Andrechek
|
| Street address |
2120 BPS Building
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE42533 |
Her2/Neu tumorigenesis and metastasis is regulated by E2F transcription factors |
|
