|
| Status |
Public on Jul 03, 2013 |
| Title |
Wild_type_Arabidopsis_11days_seedling_treated_with_JA-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Wild-type Arabidopsis thaliana 11days seedlings treated with JA
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0
genotype: wt
age: 11days
tissue: whole seedlings
treatment: jasmonate (JA)
|
| Treatment protocol |
MeJA in DMSO or DMSO only was added to liquid medium in a final concentration of 50 microM and cultured 1 hour.
|
| Growth protocol |
All plants were grown in MS (1% sucrose) liquid medium in a 16hr light / 8hr dark cycle in 23 Celsius degrees.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The Plant RNeasy Mini kit (QIAGEN) was used to isolate total RNA.
|
| Label |
Cy3
|
| Label protocol |
200 ng of total RNA was used for the labeling procedure using the Low Input Quick Amp Labeling kit (One-color) (Agilent).
|
| |
|
| Hybridization protocol |
Hybridized using the Gene Expression Hybridization kit (Agilent) and washed by the Gene Expression wash pack (Agilent).
|
| Scan protocol |
Scanned on an Agilent G2505B scanner.
|
| Description |
control_JA_rep1
Many seedlings were grown in liquid medium and were harvested for RNA extraction.
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 9.1.3.1).
Agilent Feature Extraction Software (v 9.1.3.1) was used for background subtraction. After that, the signal of each probe was divided by the median value of filter-passed probes.
|
| |
|
| Submission date |
Nov 27, 2012 |
| Last update date |
Jul 03, 2013 |
| Contact name |
Nobutaka Mitsuda |
| E-mail(s) |
nobutaka.mitsuda@aist.go.jp
|
| Organization name |
National Institute of Advanced Industrial Science and Technology
|
| Department |
Bioproduction Research Institute
|
| Lab |
Gene Regulation Research Group
|
| Street address |
Central 6 Higashi 1-1-1
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8566 |
| Country |
Japan |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE42552 |
JA-INDUCIBLE MYC2-LIKE1 acts as a repressor and negatively regulates jasmonate signaling in Arabidopsis |
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