|
| Status |
Public on Jan 30, 2013 |
| Title |
MCF-7aro (AD + Letrozole) replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
cell population of MCF-7 stably transfected with the human placental aromatase gene sensitive to letrozole (MCF-7aro cells)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF-7
|
| Treatment protocol |
Prior to experiment, cells were purged in Dulbecco’s Modified Eagle Medium without phenol red, supplemented with 3% steroid-depleted, dextran-coated and charcoal-treated fetal calf serum (DCC medium) for 4 days. MCF-7aro and Res-Let cells were then grown for 4 days in the presence of 25 nM of androstenedione combined or not with 10-6 M of letrozole. Media and treatment were changed every 2 days.
|
| Growth protocol |
Cells were grown in DMEM medium supplemented with 10% fetal bovine serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from cell culture was prepared using the miRNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations and subsequently quantified on a Nanodrop ND1000 spectrophotometer (Nanodrop Technologies, Wilmington, DE, USA). RNA integrity was checked using the BioAnalyzer 2100 (Agilent Technologies, Palo Alto, CA, USA).
|
| Label |
biotin
|
| Label protocol |
Complex probes were produced from total RNA using the FlashTag™ Biotin HSR RNA labeling kit (Affymetrix, Santa Clara, CA, USA)
|
| |
|
| Hybridization protocol |
Complex probes were hybridized to each GeneChip® miRNA 3.0 array according to the manufacturer's recommendations (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
miRNA expression data from cell population of MCF-7aro treated in the presence of 25 nM of androstenedione and 10-6M of letrozole, cell culture replicate 1
|
| Data processing |
Scanned images of microarray chips were analyzed using Expression Console (Affymetrix) with the default settings. Raw data were processed using different algorithms. Detected Above Background algorithm (DABD) and RMA Background Adjustment algorithm were performed in order to remove the background value.
|
| |
|
| Submission date |
Jan 25, 2013 |
| Last update date |
Jan 30, 2013 |
| Contact name |
Julie A Vendrell |
| E-mail(s) |
julie.vendrell@univ-lyon1.fr
|
| Organization name |
ISPBL - Faculté de Pharmacie de Lyon
|
| Department |
INSERM U1052
|
| Lab |
Centre de Recherche en Cancérologie de Lyon
|
| Street address |
8 avenue Rockefeller
|
| City |
Lyon |
| State/province |
Select a State or Province |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE43766 |
identification of miRNA differential expression patterns in a new model of acquired letrozole resistance |
|
