|
Status |
Public on Nov 03, 2013 |
Title |
Control, Fibroblasts-LacZ Replicate 2 |
Sample type |
RNA |
|
|
Source name |
FB-LacZ2
|
Organism |
Homo sapiens |
Characteristics |
cell type: Human Primary Fibroblasts treatment: transduced with LacZ expressing retrovirus
|
Treatment protocol |
Fibroblasts were transduced with LacZ or P63+KLF4. p63+KLF4 expressing cells were also treated +/- 1.2mM calcium.
|
Growth protocol |
Fibroblasts were grown in DMEM with 10% FBS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from cells was extracted using the RNeasy mini kit according to manufacturer's protocol.
|
Label |
Biotin
|
Label protocol |
Labeling and hybridization was perfomed at UCSD genechip core facility. Please refer to their website for specific details (http://www.vmrf.org/researchcenters/gene-chip/gene-chip.html)
|
|
|
Hybridization protocol |
Labeling and hybridization was perfomed at UCSD genechip core facility. Please refer to their website for specific details (http://www.vmrf.org/researchcenters/gene-chip/gene-chip.html)
|
Scan protocol |
Affymetrix HG-U133 2.0 plus arrays were performed at the UCSD Genechip Microarray Core Facility.
|
Description |
Cell transduced with LacZ expressing retrovirus
|
Data processing |
PLIER
|
|
|
Submission date |
Jun 07, 2013 |
Last update date |
Nov 03, 2013 |
Contact name |
George Sen |
E-mail(s) |
gsen@ucsd.edu
|
Organization name |
UCSD
|
Department |
Dermatology/CMM
|
Street address |
9500 GIlman Drive
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE47746 |
Gene expression data of fibroblasts transduced with LacZ or p63+KLF4. |
|