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| Status |
Public on Nov 03, 2013 |
| Title |
Gene expression data of fibroblasts transduced with LacZ or p63+KLF4. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To determine if fibroblasts could be reprogrammed to a keratinocyte phenotype p63+KLF4 or LacZ expressing retroviruses were transduced into primary human neonatal fibroblasts.
Global gene expression profiling using U133 plus 2.0 arrays were used to deteremine the extent of reprogramming to a keratinocyte phenoypte upon transduction with p63+KLF4. Fibroblasts transduced with p63+KLF4 were also treated +/- high calcium to determine if treatment with calcium could induce differentiation of these cells. Microarray analysis was also performed on cells treated +/- calcium.
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| Overall design |
For gene expression profiling, cultured human fibroblasts were infected with LacZ or p63+KLF4 expressing retroviruses. p63+KLF4 cells were also treated +/- calcium. Microarray analysis using Affymetrix HG-U133 2.0 plus arrays was performed on duplicate samples.
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| |
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| Contributor(s) |
Chen Y, Mistry DS, Sen GL |
| Citation(s) |
23921950 |
| |
| Submission date |
Jun 07, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
George Sen |
| E-mail(s) |
gsen@ucsd.edu
|
| Organization name |
UCSD
|
| Department |
Dermatology/CMM
|
| Street address |
9500 GIlman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (8)
|
| GSM1155703 |
Fibroblasts-P63+KLF4 Replicate 2 |
| GSM1155704 |
Fibroblasts-P63+KLF4 Replicate 1 (low calcium) |
| GSM1155705 |
Fibroblasts-P63+KLF4 Replicate 2 (low calcium) |
| GSM1155706 |
Fibroblasts-P63+KLF4 Replicate 1 (high calcium) |
| GSM1155707 |
Fibroblasts-P63+KLF4 Replicate 2 (high calcium) |
|
| Relations |
| BioProject |
PRJNA207551 |
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