|
| Status |
Public on Nov 03, 2013 |
| Title |
Fibroblasts-P63+KLF4 Replicate 1 (low calcium) |
| Sample type |
RNA |
| |
|
| Source name |
FB-PK1 (low calcium)
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Human Primary Fibroblasts
treatment: transduced with p63+KLF4 expressing retrovirus and treated with low calcium
|
| Treatment protocol |
Fibroblasts were transduced with LacZ or P63+KLF4. p63+KLF4 expressing cells were also treated +/- 1.2mM calcium.
|
| Growth protocol |
Fibroblasts were grown in DMEM with 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from cells was extracted using the RNeasy mini kit according to manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Labeling and hybridization was perfomed at UCSD genechip core facility. Please refer to their website for specific details (http://www.vmrf.org/researchcenters/gene-chip/gene-chip.html)
|
| |
|
| Hybridization protocol |
Labeling and hybridization was perfomed at UCSD genechip core facility. Please refer to their website for specific details (http://www.vmrf.org/researchcenters/gene-chip/gene-chip.html)
|
| Scan protocol |
Affymetrix HG-U133 2.0 plus arrays were performed at the UCSD Genechip Microarray Core Facility.
|
| Description |
Cell transduced with p63+KLF4 expressing retrovirus (low calcium)
|
| Data processing |
PLIER
|
| |
|
| Submission date |
Jun 07, 2013 |
| Last update date |
Nov 03, 2013 |
| Contact name |
George Sen |
| E-mail(s) |
gsen@ucsd.edu
|
| Organization name |
UCSD
|
| Department |
Dermatology/CMM
|
| Street address |
9500 GIlman Drive
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE47746 |
Gene expression data of fibroblasts transduced with LacZ or p63+KLF4. |
|
