|
| Status |
Public on Oct 03, 2006 |
| Title |
CD1 mouse embryonic fibroblasts |
| Sample type |
RNA |
| |
|
| Source name |
CD1 MEFs
|
| Organism |
Mus musculus |
| Characteristics |
primary mouse embryonic fibroblasts of strain CD1
|
| Treatment protocol |
inactivated CD1 MEFs incubated with basic human embryonic stem cell medium for 24h
|
| Growth protocol |
Cells were grown in FCS-containing medium, inactivated using mitomycin C, and replated onto gelatine-coated plates at 55,000 cells/sqcm. The next day, medium was changed to Serum Replacement-containing basic hES cell medium without FGF2 supplementation. RNA was isolated one day later.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit with on-column DNA digestion following the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Linear amplification kit Ambion #IL1791 following the manufacturer's instructions. Input amount: 300ng of total RNA; IVT: 6h
|
| |
|
| Hybridization protocol |
Employing materials and protocols by Illumina Inc.; hybridisation: 17h at 55 degC
|
| Scan protocol |
Using an Illumina scanner; gain factor: 2.5
|
| Description |
This sample presents the control for FGF2-treated CD1 mouse embryonic fibroblasts.
|
| Data processing |
In BeadStudio 1.0 raw data were background subtracted and normalised using the "rank invariant" algorithm. Value = "null" denotes expression below background (p < 0.01).
|
| |
|
| Submission date |
Aug 02, 2006 |
| Last update date |
Mar 21, 2008 |
| Contact name |
Boris Greber |
| E-mail(s) |
boris.greber@mpi-muenster.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Biomedicine
|
| Department |
Cell and Developmental Biology
|
| Street address |
Röntgenstraße 20
|
| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4865 |
| Series (1) |
| GSE5435 |
FGF2 stimulation experiment with CD1 mouse embryonic fibroblasts |
|
