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Sample GSM1278264 Query DataSets for GSM1278264
Status Public on Feb 23, 2014
Title Csnk1a1 knockdown (Csnk1a1-94 shRNA) in LSCs rep3
Sample type RNA
 
Source name Csnk1a1 knockdown (Csnk1a1-94 shRNA) in LSCs
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: Actin-dsRed
tissue: bone marrow
cell type: Leukemia stem cells (LSCs)
cell type info: [Hoechst low dsRed+ c-kit hi CD34+ FcyRII hi] driven by the MLL-AF9 oncogene were isolated from the bone marrow of quaternary transplant leukemic mice
Extracted molecule total RNA
Extraction protocol RNA was isolated using Trizol (Invitrogen).
Label biotin
Label protocol Standard Illumina protocol
 
Hybridization protocol Standard Illumina hybridzation protocol
Scan protocol BeadArrays were scanned with a BeadArray Reader (Illumina) and expression intensities were calculated with Beadstudio software (Illumina).
Description Csnk1a1.shRNA.3
Data processing The samples were analyzed by MouseWG-6 v2.0 Expression beadchip (Illumina). The quality of probe intensities was determined using the beadarray R package from Bioconductor (Dunning et al., 2007)
 
Submission date Dec 03, 2013
Last update date Feb 23, 2014
Contact name Fatima Al-Shahrour
E-mail(s) shahrour@broadinstitute.org
Organization name Broad Institute of MIT and Harvard
Department Cancer program
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL6887
Series (1)
GSE52928 Targeting Csnk1a1 induces selective p53-dependant killing of leukemia stem cells

Data table header descriptions
ID_REF
VALUE Quantiles algorithm
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212602 6.656115311 0.1143162
ILMN_1212603 6.592348501 0.4059829
ILMN_1212605 7.652695275 0
ILMN_1212607 6.549189951 0.633547
ILMN_1212612 6.476621287 0.8707265
ILMN_1212614 6.411650024 0.9679487
ILMN_1212619 6.733829817 0.00534188
ILMN_1212623 6.57629494 0.482906
ILMN_1212625 7.819794333 0
ILMN_1212626 6.766111024 0
ILMN_1212628 6.630272305 0.2136752
ILMN_1212632 6.717700864 0.01068376
ILMN_1212633 6.570964477 0.5042735
ILMN_1212636 8.501517609 0
ILMN_1212637 6.826589448 0
ILMN_1212638 10.47259262 0
ILMN_1212639 6.45103246 0.9241453
ILMN_1212644 6.553105643 0.6100428
ILMN_1212645 7.498214603 0
ILMN_1212646 7.587586322 0

Total number of rows: 45281

Table truncated, full table size 1469 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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