|
| Status |
Public on Feb 23, 2014 |
| Title |
Csnk1a1 knockdown (Csnk1a1-94 shRNA) in LSCs rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Csnk1a1 knockdown (Csnk1a1-94 shRNA) in LSCs
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: Actin-dsRed
tissue: bone marrow
cell type: Leukemia stem cells (LSCs)
cell type info: [Hoechst low dsRed+ c-kit hi CD34+ FcyRII hi] driven by the MLL-AF9 oncogene were isolated from the bone marrow of quaternary transplant leukemic mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridzation protocol
|
| Scan protocol |
BeadArrays were scanned with a BeadArray Reader (Illumina) and expression intensities were calculated with Beadstudio software (Illumina).
|
| Description |
Csnk1a1.shRNA.3
|
| Data processing |
The samples were analyzed by MouseWG-6 v2.0 Expression beadchip (Illumina). The quality of probe intensities was determined using the beadarray R package from Bioconductor (Dunning et al., 2007)
|
| |
|
| Submission date |
Dec 03, 2013 |
| Last update date |
Feb 23, 2014 |
| Contact name |
Fatima Al-Shahrour |
| E-mail(s) |
shahrour@broadinstitute.org
|
| Organization name |
Broad Institute of MIT and Harvard
|
| Department |
Cancer program
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE52928 |
Targeting Csnk1a1 induces selective p53-dependant killing of leukemia stem cells |
|
