|
| Status |
Public on Jan 16, 2014 |
| Title |
Human_intestinal_Metagenome_Drug-resistance_Gene_Study_175 |
| Sample type |
genomic |
| |
|
| Source name |
pre-school Children_Fecal
|
| Organism |
human gut metagenome |
| Characteristics |
gender: Femal
age: 4
molecule type: Metagenomic DNA
|
| Treatment protocol |
All the fecal samples were stored in -80°C ultra low temperature refrigerator before DNA extracton.
|
| Growth protocol |
All fecal samples were collected from healthy volunteers in Hangzhou,China. All the volunteers were required not to take any antibiotic agent in the past three months.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA from gut microbiomes was extracted directly from each fecal sample using QIAamp DNA stool mini kit according to the manufacturer’s suggested protocol.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
Group CH
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Jan 14, 2014 |
| Last update date |
Jan 16, 2014 |
| Contact name |
Yongfei Hu |
| E-mail(s) |
huyf@im.ac.cn
|
| Organization name |
Institute of Microbiology, Chinese Academy of Sciences
|
| Department |
CAS key Laboratory of Pathogenic Microbiology and Immunology
|
| Street address |
NO.1 West Beichen Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL18168 |
| Series (1) |
| GSE54070 |
Detection of antibiotic resistance genes in human gut microbiota |
|
