|
| Status |
Public on Dec 22, 2014 |
| Title |
sdg25 sdg26 seedlings Replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
sdg25 sdg26_1 seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia
organ: seedlings
age: 16 day old
genotype/variation: sdg25 sdg26
|
| Growth protocol |
Plants were grown in the greenhouse under 12h light and 12h dark at 22°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Trizol reagent (Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up according to manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
2 µg of total RNA were labeled according Aligent's instructions. Please see manufacture’s web site at http://www.aligent.com/
|
| |
|
| Hybridization protocol |
Please see manufacture’s web site at http://www.aligent.com/
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner.
Images were quantified using Agilent Feature Extraction Software.
|
| Description |
Biological replicate 1 of sdg25 sdg26 seedlings
|
| Data processing |
Agilent Feature Extraction Software was used for background subtraction. Signal intensity were normalized using Quantile method. Signal : raw Cy3 signal intensity ; Log2NormSignal : Log2 transformed Normalized Signal
|
| |
|
| Submission date |
Feb 19, 2014 |
| Last update date |
Dec 23, 2014 |
| Contact name |
Wen-Hui Shen |
| E-mail(s) |
wen-hui.shen@ibmp-cnrs.unistra.fr
|
| Phone |
+33 3 67 15 53 26
|
| Fax |
+33 3 88 61 44 42
|
| Organization name |
IBMP-CNRS
|
| Street address |
12 rue du General Zimmer
|
| City |
Strasbourg |
| ZIP/Postal code |
67084 |
| Country |
France |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE55167 |
Arabidopsis thaliana seedlings: Wild type vs. sdg26, atx1, sdg25, clf, ldl1 ldl2 and combined mutants |
|
