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Sample GSM139037 Query DataSets for GSM139037
Status Public on May 01, 2012
Title Apo_Rep2
Sample type RNA
 
Channel 1
Source name Apo_control-Replication2
Organism Oryza sativa
Characteristics Leaf samples from well-watered control plants
Treatment protocol No treatment; Well-watered control
Growth protocol Plants were grown under green house conditions and leaf samples were harvested on 37DAS.
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy5
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
Channel 2
Source name apo_Drought_replication2
Organism Oryza sativa
Characteristics Leaf samples from drought stressed plants
Treatment protocol Drought stressed for 5 days
Growth protocol Plants were grown under green house conditions and drought stress was started on 33DAS by withholding water. Leaf samples were harvested after 5 days of drought stress.
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy3
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
 
Hybridization protocol Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
Scan protocol Standard protocol of Agilent DNA Microarray Scanner

Description Keyword = rice
Keyword = Drought stress
Data processing Feature Extraction software Version 8.1 from Agilent Technologies
 
Submission date Oct 05, 2006
Last update date May 01, 2012
Contact name Raveendran Muthurajan
E-mail(s) mraveendran@irri.exch.cgiar.org, raveendrantnau@gmail.com
Phone 0063-8-536-2701
Organization name International Rice Research Institute
Department Entomology and Plant Pathology
Lab Molecular Genetics Lab
Street address Metro Manila
City makati
State/province manila
ZIP/Postal code 7777
Country Philippines
 
Platform ID GPL892
Series (1)
GSE5978 Transcript profiling of drought responsiveness in two contrasting rice genotypes during vegetative stage stress

Data table header descriptions
ID_REF
VALUE Log10 ratio of (Norm_CH2/Norm_CH1)
Norm_CH1 Normalised Intensity of Raw_CH1
Norm_CH2 Normalised Intensity of Raw_CH2
Raw_CH1 Background subtracted Intensity of CH1
Raw_CH2 Background subtracted Intensity of CH2

Data table
ID_REF VALUE Norm_CH1 Norm_CH2 Raw_CH1 Raw_CH2
1 2.15E+00 3.75E+01 5.26E+03 4.733 314.543
2 1.55E-02 5.51E+01 5.71E+01 0.900857 4.82472
3 0.00E+00 5.71E+01 3.41E+01 3.50495 0.84428
4 -2.05E-01 7.76E+01 4.84E+01 11.886 3.50094
5 1.04E-01 4.60E+01 5.84E+01 5.85879 4.37347
6 3.20E-01 5.93E+01 1.24E+02 9.5357 8.54922
7 2.24E+00 3.93E+01 6.85E+03 3.71188 409.027
8 2.69E-02 5.24E+01 5.57E+01 4.40714 4.26216
9 -3.00E-01 8.70E+03 4.36E+03 1606.92 261.405
10 9.66E-02 5.00E+01 6.25E+01 6.25944 4.63968
11 -2.43E-01 1.65E+04 9.42E+03 3006.01 572.623
12 -4.06E-02 1.28E+02 1.17E+02 21.7045 7.67355
13 0.00E+00 5.77E+01 3.48E+01 2.77679 2.26028
14 2.20E+00 3.91E+01 6.20E+03 4.94935 368.797
15 5.32E-01 1.85E+03 6.32E+03 347.972 373.111
16 1.12E-02 5.39E+01 5.53E+01 4.44234 4.23142
17 1.84E-01 5.03E+01 7.68E+01 7.40579 5.56389
18 3.88E-02 4.00E+02 4.37E+02 74.6303 25.9714
19 -7.45E-01 2.02E+05 3.63E+04 37643.5 2155.92
20 -2.32E-01 4.68E+02 2.75E+02 86.8244 16.4198

Total number of rows: 22575

Table truncated, full table size 1076 Kbytes.




Supplementary file Size Download File type/resource
GSM139037_Apo_Rep2.gpr.gz 5.2 Mb (ftp)(http) GPR

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