|
| Status |
Public on May 01, 2012 |
| Title |
IR64_Rep1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
IR64_Control_Replication1
|
| Organism |
Oryza sativa |
| Characteristics |
Leaf samples from well-watered control plants
|
| Treatment protocol |
No treatment; Well-watered control
|
| Growth protocol |
Plants were grown under green house conditions and leaf samples were harvested on 37DAS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL Reagent method
|
| Label |
Cy5
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| Channel 2 |
| Source name |
IR64_Drought_Replication1
|
| Organism |
Oryza sativa |
| Characteristics |
Leaf samples from drought stressed plants
|
| Treatment protocol |
Drought stressed for 5 days
|
| Growth protocol |
Plants were grown under green house conditions and drought stress was started on 33DAS by withholding water. Leaf samples were harvested after 5 days of drought stress.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL Reagent method
|
| Label |
Cy3
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| |
| Hybridization protocol |
Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
|
| Scan protocol |
Standard protocol of Agilent DNA Microarray Scanner
|
| Description |
Keyword = rice
Keyword = Drought stress
|
| Data processing |
Feature Extraction software Version 8.1 from Agilent Technologies
|
| |
|
| Submission date |
Oct 05, 2006 |
| Last update date |
May 01, 2012 |
| Contact name |
Raveendran Muthurajan |
| E-mail(s) |
mraveendran@irri.exch.cgiar.org, raveendrantnau@gmail.com
|
| Phone |
0063-8-536-2701
|
| Organization name |
International Rice Research Institute
|
| Department |
Entomology and Plant Pathology
|
| Lab |
Molecular Genetics Lab
|
| Street address |
Metro Manila
|
| City |
makati |
| State/province |
manila |
| ZIP/Postal code |
7777 |
| Country |
Philippines |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE5978 |
Transcript profiling of drought responsiveness in two contrasting rice genotypes during vegetative stage stress |
|
