|
Status |
Public on Oct 13, 2006 |
Title |
Testicular biopsy with Johnsen Score 2 #8 |
Sample type |
RNA |
|
|
Source name |
Testicular biopsy with Johnsen Score 2 #8
|
Organism |
Homo sapiens |
Characteristics |
Testicular biopsy with Johnsen Score 2 (Sertoli-Cell-Only syndrome)
|
Treatment protocol |
post-surgically immersed in RNALater (Ambion)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNAPure (Biozym) extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was post-purified with RNeasy columns (Qiagen).
|
Label |
Biotin-11-UTP
|
Label protocol |
Biotinylated cRNA were prepared according to Codelink protocol 080074-00 Rev. AA
|
|
|
Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 37C according to Codelink protocol 080075-00 Rev. AA
|
Scan protocol |
Arrays were scanned on an Affymetrix 428 Array Scanner at PMT gain 70
|
Description |
Testicular sandwich biopsy from a patient with SCOS, categorized by Johnsen scoring
|
Data processing |
The data were analyzed with the Codelink expression software v4.1
|
|
|
Submission date |
Oct 12, 2006 |
Last update date |
Oct 12, 2006 |
Contact name |
Andrej-Nikolai Spiess |
E-mail(s) |
a.spiess@uke.uni-hamburg.de
|
Phone |
+49-40-42803-1585
|
Fax |
+49-40-42803-1554
|
URL |
http://www.dr-spiess.de
|
Organization name |
University Hospital Hamburg-Eppendorf
|
Department |
Andrology
|
Lab |
Molecular Andrology
|
Street address |
Martinistr. 52
|
City |
20246 Hamburg |
State/province |
n/a |
ZIP/Postal code |
n/a |
Country |
Germany |
|
|
Platform ID |
GPL2891 |
Series (1) |
GSE6023 |
Expression data of testis biopsies obtained from men with spermatogenic impairment |
|