|
| Status |
Public on Oct 13, 2006 |
| Title |
Testicular biopsy with Johnsen Score 5 #6 |
| Sample type |
RNA |
| |
|
| Source name |
Testicular biopsy with Johnsen Score 5 #6
|
| Organism |
Homo sapiens |
| Characteristics |
Testicular biopsy with Johnsen Score 5 (pre/meiotic arrest)
|
| Treatment protocol |
post-surgically immersed in RNALater (Ambion)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAPure (Biozym) extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was post-purified with RNeasy columns (Qiagen).
|
| Label |
Biotin-11-UTP
|
| Label protocol |
Biotinylated cRNA were prepared according to Codelink protocol 080074-00 Rev. AA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 37C according to Codelink protocol 080075-00 Rev. AA
|
| Scan protocol |
Arrays were scanned on an Affymetrix 428 Array Scanner at PMT gain 70
|
| Description |
Testicular sandwich biopsy from a patient with pre/meiotic arrest, categorized by Johnsen scoring
|
| Data processing |
The data were analyzed with the Codelink expression software v4.1
|
| |
|
| Submission date |
Oct 12, 2006 |
| Last update date |
Oct 12, 2006 |
| Contact name |
Andrej-Nikolai Spiess |
| E-mail(s) |
a.spiess@uke.uni-hamburg.de
|
| Phone |
+49-40-42803-1585
|
| Fax |
+49-40-42803-1554
|
| URL |
http://www.dr-spiess.de
|
| Organization name |
University Hospital Hamburg-Eppendorf
|
| Department |
Andrology
|
| Lab |
Molecular Andrology
|
| Street address |
Martinistr. 52
|
| City |
20246 Hamburg |
| State/province |
n/a |
| ZIP/Postal code |
n/a |
| Country |
Germany |
| |
|
| Platform ID |
GPL2891 |
| Series (1) |
| GSE6023 |
Expression data of testis biopsies obtained from men with spermatogenic impairment |
|
