|
| Status |
Public on Dec 30, 2015 |
| Title |
mammary_gland_tumor-Elf5_EGFP_pos rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary gland tumor, Elf5 dox induced EGFP positive
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
tissue: mammary gland tumor
strain: FBV/N
genotype/variation: chronic (8 weeks) Elf5 induced expression
|
| Treatment protocol |
Mice were exposed continuosly to a diet containing 700mg/Kg of Doxocyclin (Gordon’s Specialty Stockfeeds) from week 6 of age until the ethical endpoint (8 weeks total treatment)
|
| Growth protocol |
MMTV-PyMT mouse mammary tumor samples (and metastasis) were processed for single cell suspension and FACS sorted based on Lin- CD24+ markers. RNA was extracted from these populations straight after FACS sorting.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by using the Qiagen RNeasy minikit (Qiagen, Doncaster, Vic, Australia) according to manufacturer’s protocol. Following extraction, RNA concentration and purity were analyzed using the NanoDrop® ND-1000 Spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA). For Affymetrix GeneChip® analysis, the integrity of RNA was analyzed using the Agilent Bioanalyser 2100 6000 Nano Assay (Agilent Technologies, Palo Alto, CA, USA) according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Total RNA was converted to cDNA. The cDNA was fragmented, biotin labeled at the Ramaciotti Centre for Gene Function Analysis at the University of New South Wales (UNSW, Sydney, NSW, Australia), following manufacturers instructions.
|
| |
|
| Hybridization protocol |
Labeled, fragmented cDNA hybridized to Affymetrix Mouse Gene 1.0 ST Gene Arrays at the Ramaciotti Centre for Gene Function Analysis at the University of New South Wales (UNSW, Sydney, NSW, Australia), following manufacturers instructions.
|
| Scan protocol |
Arrays were scanned at the Ramaciotti Centre for Gene Function Analysis at the University of New South Wales (UNSW, Sydney, NSW, Australia), following manufacturers instructions.
|
| Description |
Mammary gland tumor, Elf5 dox induced EGFP positive
GFP_Pos_3
|
| Data processing |
Data was RMA normalized using NormalizeAffymetrixST (version 2.0) GenePattern module from the Peter Wills Bioinformatics Centre's GenePattern Server: http://pwbc.garvan.unsw.edu.au/gp
|
| |
|
| Submission date |
Jun 23, 2014 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Daniel Roden |
| E-mail(s) |
d.roden@garvan.org.au
|
| Organization name |
Garvan Institute
|
| Street address |
384 Victoria Street
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE58728 |
The ETS transcription factor Elf5 drives lung metastasis in luminal breast cancer via recruitment of Gr-1+CD11b+ myeloid derived suppressor cells [chronic] |
| GSE58729 |
The ETS transcription factor Elf5 drives lung metastasis in luminal breast cancer via recruitment of Gr-1+CD11b+ myeloid derived suppressor cells |
|
