|
| Status |
Public on Oct 25, 2007 |
| Title |
RDA RSV 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
NIL37 RSV 1
|
| Organism |
Oryza sativa |
| Characteristics |
leaves of 14-day old seedling
|
| Treatment protocol |
Leaves of 7-day old seedlings were inoculated 3 small brown planthoppers carrying rice stripe virus for 24 hours. Leaves were harvested 7 days after inoculation.
|
| Growth protocol |
Seedlings were grown in a greenhouse for 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol according to the manufaturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA input fluorescent linear amplification kit according to the manufacturer's instrcution.
|
| |
|
| Channel 2 |
| Source name |
NIL22 RSV 1
|
| Organism |
Oryza sativa |
| Characteristics |
leaves of 14-day old seedling
|
| Treatment protocol |
Leaves of 7-day old seedlings were inoculated 3 small brown planthoppers carrying rice stripe virus for 24 hours. Leaves were harvested 7 days after inoculation.
|
| Growth protocol |
Seedlings were grown in a greenhouse for 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol according to the manufaturer's instruction.
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA input fluorescent linear amplification kit according to the manufacturer's instrcution.
|
| |
|
| |
| Hybridization protocol |
in-situ Hybridization Kit Plus (Agilent Technologies) according to the manufacturer's instrcution.
|
| Scan protocol |
DNA microarray scanner (Agilent Technologies) according to the manufacturer's instrcution.
|
| Description |
NIL37 and NIL22 were sister NIL (BC4) derived from the cross between varieties Ilpum (recurrent parent) and Shinkwang (donor parent). NIL37 is susceptible to rice stripe virus (RSV), and NIL22 is resistant to RSV.
|
| Data processing |
Feature Extraction Vert. 8.1 (Agilent Technologies)
|
| |
|
| Submission date |
Oct 25, 2006 |
| Last update date |
Nov 06, 2006 |
| Contact name |
IL-RYONG CHOI |
| E-mail(s) |
ichoi@cgiar.org
|
| Organization name |
International Rice Research Institute
|
| Street address |
DAPO Box 7777
|
| City |
Metro Manila |
| ZIP/Postal code |
0000 |
| Country |
Philippines |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE6126 |
Comparative gene expression analysis between virus-susceptible and -resistant rice lines (NIL37 and NIL22) |
|
