|
| Status |
Public on Oct 31, 2009 |
| Title |
P-def_rep2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
6-4_P-def_rep2; without Phosphorus
|
| Organism |
Oryza sativa |
| Characteristics |
root tissues sampled in 40 day-old plants
|
| Growth protocol |
Plants were grown in 12L pots filled with soil (Andosol) without Phosphorus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of root tissues (1.6 g) was collected and purified by RNeasy midi kit according to manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Labelled cRNA was synthesized by Low RNA Input Linear Amplification kit (Agilent Technlogies) according to manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
Nipponbare_P-def_rep2; without Phosphorus
|
| Organism |
Oryza sativa |
| Characteristics |
root tissues sampled in 40 day-old plants
|
| Growth protocol |
Plants were grown in 12L pots filled with soil (Andosol) without Phosphorus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of root tissues (1.6 g) was collected and purified by RNeasy midi kit according to manufacturer's protocol.
|
| Label |
Cy5
|
| Label protocol |
Labelled cRNA was synthesized by Low RNA Input Linear Amplification kit (Agilent Technlogies) according to manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
Both 1 micro gram labelled cRNA were hybridized on a slide by In-situ Hybridization Kit Plus (Agilent Technologies) according to manufacturer's protocol.
|
| Scan protocol |
After hybridization, the slide was washed twice according to manufacturer's protocol. the slide was scanned by DNA microarray scaner according to manufacturer's protocol.
|
| Description |
Keyword: Nipponbare
Keyword: 6-4 (NIL with inserts from cv. Kasalath (indica))
Keyword: Comparison between line
Keyword: Phosphorus deficiency
Keyword: Root
|
| Data processing |
Feature Extraction ver 8.1 (Agilent Technologies)
|
| |
|
| Submission date |
Oct 26, 2006 |
| Last update date |
Mar 24, 2009 |
| Contact name |
Matthias Wissuwa |
| E-mail(s) |
wissuwa@affrc.go.jp
|
| Phone |
+81-29-838-6354
|
| Fax |
+81-29-838-6354
|
| Organization name |
JIRCAS
|
| Street address |
1-1 Ohwashi
|
| City |
Tsukuba |
| ZIP/Postal code |
305-8686 |
| Country |
Japan |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE6187 |
Comparative gene expression analysis between Nipponbare and 6-4 under two conditions |
|
