|
Status |
Public on Oct 31, 2009 |
Title |
P-def_rep3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Nipponbare_P-def_rep3; without Phosphorus
|
Organism |
Oryza sativa |
Characteristics |
root tissues sampled in 40 day-old plants
|
Growth protocol |
Plants were grown in 12L pots filled with soil (Andosol) without Phosphorus
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA of root tissues (1.6 g) was collected and purified by RNeasy midi kit according to manufacturer's protocol.
|
Label |
Cy3
|
Label protocol |
Labelled cRNA was synthesized by Low RNA Input Linear Amplification kit (Agilent Technlogies) according to manufacturer's protocol.
|
|
|
Channel 2 |
Source name |
6-4_P-def_rep3; without Phosphorus
|
Organism |
Oryza sativa |
Characteristics |
root tissues sampled in 40 day-old plants
|
Growth protocol |
Plants were grown in 12L pots filled with soil (Andosol) without Phosphorus
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA of root tissues (1.6 g) was collected and purified by RNeasy midi kit according to manufacturer's protocol.
|
Label |
Cy5
|
Label protocol |
Labelled cRNA was synthesized by Low RNA Input Linear Amplification kit (Agilent Technlogies) according to manufacturer's protocol.
|
|
|
|
Hybridization protocol |
Both 1 micro gram labelled cRNA were hybridized on a slide by In-situ Hybridization Kit Plus (Agilent Technologies) according to manufacturer's protocol.
|
Scan protocol |
After hybridization, the slide was washed twice according to manufacturer's protocol. the slide was scanned by DNA microarray scaner according to manufacturer's protocol.
|
Description |
Keyword: Nipponbare Keyword: 6-4 (NIL with inserts from cv. Kasalath (indica)) Keyword: Comparison between line Keyword: Phosphorus deficiency Keyword: Root
|
Data processing |
Feature Extraction ver 8.1 (Agilent Technologies)
|
|
|
Submission date |
Oct 26, 2006 |
Last update date |
Mar 24, 2009 |
Contact name |
Matthias Wissuwa |
E-mail(s) |
wissuwa@affrc.go.jp
|
Phone |
+81-29-838-6354
|
Fax |
+81-29-838-6354
|
Organization name |
JIRCAS
|
Street address |
1-1 Ohwashi
|
City |
Tsukuba |
ZIP/Postal code |
305-8686 |
Country |
Japan |
|
|
Platform ID |
GPL892 |
Series (1) |
GSE6187 |
Comparative gene expression analysis between Nipponbare and 6-4 under two conditions |
|