|
| Status |
Public on Aug 02, 2016 |
| Title |
Biopsy Liver sample: CA.82_.miRNA.3_0..CEL |
| Sample type |
RNA |
| |
|
| Source name |
Liver sample
|
| Organism |
Homo sapiens |
| Characteristics |
immunotolerance group: AH
disease state: Alcoholic hepatitis
tissue: liver
|
| Treatment protocol |
n/a
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from liver tissues using Trizol and following manufacter’s manual instructions (Life Tecnologies, Carlsbad, CA). miRNA quantification was performed using the commercial kit Mir-X miRNA First-Strand Synthesis Kit doing as manufacter’s manual instructions (Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France).
|
| Label |
biotin
|
| Label protocol |
Biotin
|
| |
|
| Hybridization protocol |
Complex probes were hybridized to each GeneChip® miRNA 3.0 array according to the manufacturer's recommendations (Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
AH Biopsy Liver sample: CA.82_.miRNA.3_0..CEL
|
| Data processing |
Scanned images of microarray chips were analyzed using Expression Console (Affymetrix) with the default settings. Raw data were processed using different algorithms. Detected Above Background algorithm (DABD) and RMA Background Adjustment algorithm were performed in order to remove the background value.
|
| |
|
| Submission date |
Jul 16, 2014 |
| Last update date |
Aug 02, 2016 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
juanjo.lozano@ciberehd.org
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE59492 |
Integrative analysis of microRNA and gene expression profiles identifies microRNAs as potential regulators in alcoholic hepatitis |
|
