|
| Status |
Public on Oct 29, 2007 |
| Title |
Inoculated 120 hours KR12002 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
120_Ajaya_Replication 2
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
Leaf samples from inoculated plants of Ajaya collected at 120 hrs after inoculation
|
| Treatment protocol |
Leaves were inoculated with Xanthomonas oryzae pv oryzae by clip inoculation method (inoculated)
|
| Growth protocol |
Plants were grown under Growth chambers and leaf samples were harvested on 23 DAS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen Rneasy Plant Maxi Kit according to manufacturers protocol
|
| Label |
Cy5
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| Channel 2 |
| Source name |
120_IR24_Replication 2
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
Leaf samples from inoculated plants of IR24 collected at 120 hrs after inoculation
|
| Treatment protocol |
Leaves were inoculated with Xanthomonas oryzae pv oryzae by clip inoculation method (inoculated)
|
| Growth protocol |
Plants were grown under Growth chambers and leaf samples were harvested on 23 DAS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Qiagen Rneasy Plant Maxi Kit according to manufacturers protocol
|
| Label |
Cy3
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| |
| Hybridization protocol |
Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
|
| Scan protocol |
Standard protocol of Agilent DNA Microarray Scanner
|
| Description |
Keyword = rice
Keyword = bacterial leaf blight disease response
|
| Data processing |
Feature Extraction software Version 8.1 from Agilent Technologies
|
| |
|
| Submission date |
Nov 08, 2006 |
| Last update date |
Oct 29, 2007 |
| Contact name |
Kameswara Rao Kottapalli |
| E-mail(s) |
Kottapallisri@gmail.com
|
| Organization name |
USDA-ARS Lubbock & Texas Tech University
|
| Department |
Plant & Soil Science
|
| Lab |
Payton Lab
|
| Street address |
3810 4th Street
|
| City |
Lubbock |
| State/province |
TX |
| ZIP/Postal code |
79415 |
| Country |
USA |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE6244 |
Transcriptional profiling of indica rice cultivar IET8585 (Ajaya) infected with bacterial leaf blight pathogen (Xoo) |
|
