|
Status |
Public on Oct 29, 2007 |
Title |
Inoculated 120 hours KR12002 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
120_Ajaya_Replication 2
|
Organism |
Oryza sativa Indica Group |
Characteristics |
Leaf samples from inoculated plants of Ajaya collected at 120 hrs after inoculation
|
Treatment protocol |
Leaves were inoculated with Xanthomonas oryzae pv oryzae by clip inoculation method (inoculated)
|
Growth protocol |
Plants were grown under Growth chambers and leaf samples were harvested on 23 DAS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Qiagen Rneasy Plant Maxi Kit according to manufacturers protocol
|
Label |
Cy5
|
Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
|
|
Channel 2 |
Source name |
120_IR24_Replication 2
|
Organism |
Oryza sativa Indica Group |
Characteristics |
Leaf samples from inoculated plants of IR24 collected at 120 hrs after inoculation
|
Treatment protocol |
Leaves were inoculated with Xanthomonas oryzae pv oryzae by clip inoculation method (inoculated)
|
Growth protocol |
Plants were grown under Growth chambers and leaf samples were harvested on 23 DAS.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Qiagen Rneasy Plant Maxi Kit according to manufacturers protocol
|
Label |
Cy3
|
Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
|
|
|
Hybridization protocol |
Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
|
Scan protocol |
Standard protocol of Agilent DNA Microarray Scanner
|
Description |
Keyword = rice Keyword = bacterial leaf blight disease response
|
Data processing |
Feature Extraction software Version 8.1 from Agilent Technologies
|
|
|
Submission date |
Nov 08, 2006 |
Last update date |
Oct 29, 2007 |
Contact name |
Kameswara Rao Kottapalli |
E-mail(s) |
Kottapallisri@gmail.com
|
Organization name |
USDA-ARS Lubbock & Texas Tech University
|
Department |
Plant & Soil Science
|
Lab |
Payton Lab
|
Street address |
3810 4th Street
|
City |
Lubbock |
State/province |
TX |
ZIP/Postal code |
79415 |
Country |
USA |
|
|
Platform ID |
GPL892 |
Series (1) |
GSE6244 |
Transcriptional profiling of indica rice cultivar IET8585 (Ajaya) infected with bacterial leaf blight pathogen (Xoo) |
|