|
| Status |
Public on Nov 24, 2007 |
| Title |
KS15R02 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
15 min_IR24_Replication 2
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
Leaf samples from submerged plants of IR24 collected after 15 min of submergence
|
| Treatment protocol |
14 days old plants were submerged in transparent polythene bags in growth chamber
|
| Growth protocol |
Plants were grown in Growth chambers and leaf samples were harvested on 14 DAS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Plant Maxi Kit and according to manufacturers protocol.
|
| Label |
Cy5
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| Channel 2 |
| Source name |
15 min_FR13A_Replication 2
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
Leaf samples from submerged plants of FR13A collected after 15 min of submergence
|
| Treatment protocol |
14 days old plants were submerged in transparent polythene bags in growth chamber
|
| Growth protocol |
Plants were grown in Growth chambers and leaf samples were harvested on 14 DAS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Plant Maxi Kit and according to manufacturers protocol.
|
| Label |
Cy3
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
|
| |
|
| |
| Hybridization protocol |
Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
|
| Scan protocol |
Standard protocol of Agilent DNA Microarray Scanner
|
| Description |
Keyword = rice
Keyword = submergence stress response
|
| Data processing |
Feature Extraction software Version 8.1 from Agilent Technologies
|
| |
|
| Submission date |
Nov 24, 2006 |
| Last update date |
Nov 27, 2006 |
| Contact name |
Kameswara Rao Kottapalli |
| E-mail(s) |
Kottapallisri@gmail.com
|
| Organization name |
USDA-ARS Lubbock & Texas Tech University
|
| Department |
Plant & Soil Science
|
| Lab |
Payton Lab
|
| Street address |
3810 4th Street
|
| City |
Lubbock |
| State/province |
TX |
| ZIP/Postal code |
79415 |
| Country |
USA |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE6362 |
Transcriptional profiling of indica rice cultivar FR13A under submergence treatment. |
|
