|
| Status |
Public on Oct 03, 2014 |
| Title |
MMTV-Cre:Rbp53 primary tumor TMCRP-1123 |
| Sample type |
RNA |
| |
|
| Source name |
Primary mammary tumor Rbp53 deleted
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed
tumor model: MMTV-Cre (Transplant)
tissue: Primary mammary tumor Rbp53 deleted
|
| Treatment protocol |
Total RNA obtained from primary tumors and normal mammary tissue were used for microarray analysis to determine differentially regulated genes. Cluster analysis and pathway analysis based on gene expression were then performed to determine significant genes/pathways
|
| Growth protocol |
Samples from independent primary mammary tumors from MMTV-Cre:Rbp53,MMTV-Cre:p53, Ad-Cre:Rbp53 models and normal mammary tissue were frozen and kept in -80 degree.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent twice. Quality control was performed with Agilent Bioanalyser
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| |
|
| Hybridization protocol |
standard Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Scan protocol |
standard Affymetrix Mouse Gene 1.0 st microarray experiment were performed by the Centre of Applied Genomics (HSC, Toronto)
|
| Description |
gene expression data from Rbp53 deleted mammary tumor
|
| Data processing |
Microarray Data were normalized using RMA method via Partek Genomics Suite (v.5.6) and absolute expression values were obtained
|
| |
|
| Submission date |
Oct 02, 2014 |
| Last update date |
Oct 03, 2014 |
| Contact name |
Robert Jones |
| E-mail(s) |
rjones12@uoguelph.ca
|
| Organization name |
University of Guelph
|
| Department |
Biomedical Sciences
|
| Street address |
50 Stone Rd
|
| City |
Guelph |
| ZIP/Postal code |
N1G 2W1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE62016 |
Expression data comparing Rb/p53 and p53 deficient mammary tumors and normal mammary tissue |
|
