|
| Status |
Public on Feb 20, 2007 |
| Title |
Primary prostate tumor 04_0014_Uniset20K |
| Sample type |
RNA |
| |
|
| Source name |
04_0014
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue:primary prostate tumor
|
| Treatment protocol |
Fresh prostate tissues were recovered immediately after surgical removal. Tissues were dissected/trimmed to obtain pure tumor then snap frozen in liquid nitrogen within 30 mintues of excision and stored in -80C untill RNA extraction. An H&E stained frozen section of each sample was evaluated by a pathologist, to determine epithelial and stromal content and verify the presence of tumor in the sample. Only tumor tissues with less than 30% of tromal components were selected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard CodeLink protocol from 1 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 18 hr at 37C on Human Uniset 20K arrays (GE Healthcare, Piscataway, NJ). Arrays were washed and stained according to the standard CodeLink manual.
|
| Scan protocol |
CodeLink arrays were scanned with the GenePix 4000B scanner using GenePix Pro 4.1 software.
|
| Description |
CodeLink gene expression data from normal prostate tissues free of any pathological alteration
|
| Data processing |
For CodeLink arrays, image files were analyzed with the CodeLink Expression Analysis Software version 4.1 (GE HealthCare) with use of the normalized intensity values in downstream analysis.
|
| |
|
| Submission date |
Jan 16, 2007 |
| Last update date |
Feb 20, 2007 |
| Contact name |
Federico Alberto Monzon |
| E-mail(s) |
famonzon@tmhs.org
|
| Organization name |
The Methodist Hospital
|
| Department |
Pathology
|
| Lab |
Molecular Diagnostics
|
| Street address |
6565 Fannin St, MS205
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL2891 |
| Series (1) |
| GSE6752 |
CodeLink Expression Data from primary prostate tumor and prostate tumor metastases |
|
