|
| Status |
Public on Jul 13, 2015 |
| Title |
aortic tissue replicate 1 |
| Sample type |
SRA |
| |
|
| Source name |
Aorta
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: aorta
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue homogenization and RNA extraction by Qiagen mini-column
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Base calls were performed using the built-in Illumina basecaller
Sequenced reads were trimmed for adaptor sequence, and then mapped to Rat Rnor5.72 reference using the TopHat splice-aware aligner
Reads Per Kilobase of gene per Million mapped reads (RPKM) were calculated for each sample.
Genome_build: R. norvegicus (Rnor5.72)
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each sample
|
| |
|
| Submission date |
Dec 22, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Amol Carl Shetty |
| E-mail(s) |
ashetty@som.umaryland.edu
|
| Organization name |
University of Maryland Baltimore
|
| Department |
Institute for Genome Sciences
|
| Street address |
670 W. Baltimore Street, Floor 3
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL14844 |
| Series (1) |
| GSE64450 |
Unique gene program of rat mesenteric arteries as revealed by Deep RNA Sequencing |
|
| Relations |
| BioSample |
SAMN03272459 |
| SRA |
SRX821635 |
