|
| Status |
Public on Mar 31, 2016 |
| Title |
ColInf2dpi1.txt:gProcessedSignal |
| Sample type |
RNA |
| |
|
| Source name |
fully expanded leaves_Col-0_infected_2.5
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: Col-0
tissue: fully expanded leaves
infection status: infected
pathogen: Colletotrichum higginsianum
time point: 2.5 dpi
|
| Treatment protocol |
Five weeks old Arabidopsis plants were spray infected 1h before the end of the light period with 2 Mio. conidia/ ml of Colletotrichum higginsianum isolate MAFF 305635 harvested from 9d old OMA plates in a humid tray sealed with moist towel paper and covered with a dome. These high humidity conditions were released at 62 hours post treatment
|
| Growth protocol |
Arabidopsis plants were germinated for 10 days in short day conditions at 21°C and subsequently grown in a phytochamber in 12h (22°C)/ 12h (20°C) light-dark cycle for 25 days. At day 18 in the phytochamber, plants were fertilized with 40ml of a 0.2% Wuxal Super solution (Aglykon, Düsseldorf).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total leaf RNA was extracted using a guanidium isothiocyanate based method (as described by Chomczynski and Sacchi, 1987).
|
| Label |
Cy3
|
| Label protocol |
Cy3-labeled cRNA were prepared according to the agilent protocol from 400ng total RNA (One-Color Microarray-based gene expression analysis v.5.5)
|
| |
|
| Hybridization protocol |
following fragmentation, 600ng of Cy3-labeled cRNA were hybridisied for 17h at 65°C on Arabidopsis Agilent AthV4 arrays (Agilent Design ID 021169). Arrays were washed according to the protocol (One-Color Microarray-based gene expression analysis v.5.5)
|
| Scan protocol |
Microarrays were scanned using Agilent G2565B Microarray Scanner. Data were extracted using Feature Extraction Version: FE Version 9.5.3.1 (Agilent Technologies Germany).
|
| Data processing |
Data analysis was performed using GeneSpringXI (Agilent Technologies)
Signal intensities were normalized to the 75th percentile and normalized data were baseline transformed to the median using Gene Spring XI software.
|
| |
|
| Submission date |
Apr 02, 2015 |
| Last update date |
Mar 31, 2016 |
| Contact name |
Lars Matthias Voll |
| E-mail(s) |
lars.voll@fau.de
|
| Phone |
+49 (0)9131 8525238
|
| Organization name |
Friedrich-Alexander-University Erlangen-Nuremberg
|
| Department |
Division of Biochemistry
|
| Street address |
Staudtstrasse 5
|
| City |
Erlangen |
| ZIP/Postal code |
91058 |
| Country |
Germany |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE67544 |
Transcriptional response of Arabidopsis sweet11/sweet12 double mutants upon Colletotrichum higginsianum infection |
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