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Status |
Public on Mar 31, 2016 |
Title |
SWEETMock2dpi1.txt:gProcessedSignal |
Sample type |
RNA |
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Source name |
fully expanded leaves_sweet11/sweet12_mock_2.5
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Organism |
Arabidopsis thaliana |
Characteristics |
genotype: sweet11/sweet12 tissue: fully expanded leaves infection status: mock pathogen: none time point: 2.5 dpi
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Treatment protocol |
Five weeks old Arabidopsis plants were spray infected 1h before the end of the light period with 2 Mio. conidia/ ml of Colletotrichum higginsianum isolate MAFF 305635 harvested from 9d old OMA plates in a humid tray sealed with moist towel paper and covered with a dome. These high humidity conditions were released at 62 hours post treatment
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Growth protocol |
Arabidopsis plants were germinated for 10 days in short day conditions at 21°C and subsequently grown in a phytochamber in 12h (22°C)/ 12h (20°C) light-dark cycle for 25 days. At day 18 in the phytochamber, plants were fertilized with 40ml of a 0.2% Wuxal Super solution (Aglykon, Düsseldorf).
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Extracted molecule |
total RNA |
Extraction protocol |
Total leaf RNA was extracted using a guanidium isothiocyanate based method (as described by Chomczynski and Sacchi, 1987).
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Label |
Cy3
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Label protocol |
Cy3-labeled cRNA were prepared according to the agilent protocol from 400ng total RNA (One-Color Microarray-based gene expression analysis v.5.5)
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Hybridization protocol |
following fragmentation, 600ng of Cy3-labeled cRNA were hybridisied for 17h at 65°C on Arabidopsis Agilent AthV4 arrays (Agilent Design ID 021169). Arrays were washed according to the protocol (One-Color Microarray-based gene expression analysis v.5.5)
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Scan protocol |
Microarrays were scanned using Agilent G2565B Microarray Scanner. Data were extracted using Feature Extraction Version: FE Version 9.5.3.1 (Agilent Technologies Germany).
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Data processing |
Data analysis was performed using GeneSpringXI (Agilent Technologies) Signal intensities were normalized to the 75th percentile and normalized data were baseline transformed to the median using Gene Spring XI software.
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Submission date |
Apr 02, 2015 |
Last update date |
Mar 31, 2016 |
Contact name |
Lars Matthias Voll |
E-mail(s) |
lars.voll@fau.de
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Phone |
+49 (0)9131 8525238
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Organization name |
Friedrich-Alexander-University Erlangen-Nuremberg
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Department |
Division of Biochemistry
|
Street address |
Staudtstrasse 5
|
City |
Erlangen |
ZIP/Postal code |
91058 |
Country |
Germany |
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Platform ID |
GPL12621 |
Series (1) |
GSE67544 |
Transcriptional response of Arabidopsis sweet11/sweet12 double mutants upon Colletotrichum higginsianum infection |
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