|
| Status |
Public on Apr 17, 2019 |
| Title |
Undifferentiated-Differentiated GSCs Sample 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
glioblastoma stem cells-2
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: Brain
cell type: undifferentiated GSC
grade: WHO Ⅳ
|
| Treatment protocol |
GSCs differentiation: after 5 passages in GSCs-propagating medium, GSCs were re-suspended and cultured in DMEM/F12 medium containing 10% fetal bovine serum (FBS) (Gibco, Australia) and without N2 and B27 for 4 days
|
| Growth protocol |
GSCs were re-suspended and maintained in GSCs-propagating medium composed of DMEM/F-12 medium, 2 mM GlutaMAX (Invitrogen, USA), 20 ng/mL recombinant human epidermal growth factor (EGF) (R&D, USA), 20 ng/mL basic fibroblast growth factor (bFGF) (Invitrogen, USA), N2 supplement (Invitrogen, USA), and B27 supplement (Invitrogen, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
To see manufacturer's website http://bioservices.capitalbio.com/fwpt/Agilentpt/15026.shtml
|
| |
|
| Channel 2 |
| Source name |
differentiated glioblastoma stem cells-2
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: Brain
cell type: differentiated GSC
development stage: WHO Ⅳ
|
| Treatment protocol |
GSCs differentiation: after 5 passages in GSCs-propagating medium, GSCs were re-suspended and cultured in DMEM/F12 medium containing 10% fetal bovine serum (FBS) (Gibco, Australia) and without N2 and B27 for 4 days
|
| Growth protocol |
GSCs were re-suspended and maintained in GSCs-propagating medium composed of DMEM/F-12 medium, 2 mM GlutaMAX (Invitrogen, USA), 20 ng/mL recombinant human epidermal growth factor (EGF) (R&D, USA), 20 ng/mL basic fibroblast growth factor (bFGF) (Invitrogen, USA), N2 supplement (Invitrogen, USA), and B27 supplement (Invitrogen, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
To see manufacturer's website http://bioservices.capitalbio.com/fwpt/Agilentpt/15026.shtml
|
| |
|
| |
| Hybridization protocol |
To see manufacturer's website http://bioservices.capitalbio.com/fwpt/Agilentpt/15026.shtml
|
| Scan protocol |
To see manufacturer's website http://bioservices.capitalbio.com/fwpt/Agilentpt/15026.shtml
|
| Description |
UNDE-DE-GSCs-2
Biological replicate 3 of 4. undifferentiated glioblastoma stem cells vs. differentiated glioblastoma stem cells
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version A.7.5).
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Apr 28, 2015 |
| Last update date |
Apr 17, 2019 |
| Contact name |
Hao Li |
| E-mail(s) |
handanlihao-1@163.com
|
| Organization name |
Beijing Tiantan Hospital, Capital Medical University
|
| Department |
Department of Neurosurgery
|
| Street address |
No.6 Tiantanxili
|
| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE68343 |
Human glioblastoma stem cells (GSCs) differentiation: Undifferentiated cells vs. Differentiated cells |
|
