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Sample GSM182342 Query DataSets for GSM182342
Status Public on Apr 01, 2008
Title Self-Hyb_shoot8
Sample type RNA
 
Channel 1
Source name 10 days old shoot8
Organism Oryza sativa Japonica Group
Characteristics cultivar: Nipponbare
Tissue: shoot
stage: LP.03 three leaves visible
age: 10 days after sowing
Growth protocol growth: hydroponic culture
growth enviroment: growth chamber study
Growth substrates: 1/5000 diluted Hyponex (10-3-3) solution
light condition: 16hrs day length
temperature: 28 degreeC
Extracted molecule total RNA
Extraction protocol Separation technique: Trimming
Extraction source: Frozen
Kit: RNeasy Midi Kit
Manufacturer: QIAGEN
Manual: QIAGEN
Kit: OligotexTM-dt30 <Super> mRNA Purification kit (From Total RNA)
Manufacturer: TaKaRa
Manual: TaKaRa
Label Cy3
Label protocol Kit: Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Linear Amplification kit standard protocol
 
Channel 2
Source name 10 days old shoot8
Organism Oryza sativa Japonica Group
Characteristics cultivar: Nipponbare
Tissue: shoot
stage: LP.03 three leaves visible
age: 10 days after sowing
Growth protocol growth: hydroponic culture
growth enviroment: growth chamber study
Growth substrates: 1/5000 diluted Hyponex (10-3-3) solution
light condition: 16hrs day length
temperature: 28 degreeC
Extracted molecule total RNA
Extraction protocol Separation technique: Trimming
Extraction source: Frozen
Kit: RNeasy Midi Kit
Manufacturer: QIAGEN
Manual: QIAGEN
Kit: OligotexTM-dt30 <Super> mRNA Purification kit (From Total RNA)
Manufacturer: TaKaRa
Manual: TaKaRa
Label Cy5
Label protocol Kit: Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Linear Amplification kit standard protocol
 
 
Hybridization protocol hybridization_Kit: in situ hybridization plus
hybridization_Manufacturer: Agilent Technologies
hybridization_Manual: Agilent Technologies
Scan protocol Scanner: Agilent DNA microarray Scaner
Manufacturer: Agilent Technologies
Software: Scanner Control software
Manufacturer: Agilent Technologies
Manual: Agilent Technologies
Description Technical replicate 1 of 4., self-hybridization
Data processing Software: Feature Extraction ver 8.1.1.1
Manufacturer: Agilent Technologies
Manual: Agilent Technologies
 
Submission date Apr 16, 2007
Last update date Apr 16, 2007
Contact name Kouji Satoh
Organization name NARO/HARC
Street address Hitsujigaoka 1
City Sapporo
State/province Hokkaido
ZIP/Postal code 062-8555
Country Japan
 
Platform ID GPL892
Series (1)
GSE7530 Gene expression profiles of various tissues under different physiological conditions

Data table header descriptions
ID_REF
VALUE log10 ratio (Ch2 normalized signal intensity / Ch1 normalized signal intensity)
PValueLogRatio Significance level of the Log Ratio computed for a feature
gBGSubSignal The net CH1 signal following the subtraction of the background from the raw mean CH1 signal
rBGSubSignal The net CH2 signal following the subtraction of the background from the raw mean CH2 signal

Data table
ID_REF VALUE PValueLogRatio gBGSubSignal rBGSubSignal
1 0.005015569 0.92639873 583.309 1175.41
2 1 -12.4792 -31.176
3 0.011717431 0.904345962 158.579 325.251
4 0.018393659 0.986847692 12.0742 24.3791
5 0.002308707 0.974150946 261.624 525.197
6 -0.083779604 0.841403466 36.0259 58.5649
7 -0.006437679 0.922449807 308.227 605.91
8 0.010769931 0.878280488 265.756 543.954
9 0.036278323 0.486989327 746.11 1619.03
10 -0.058591573 0.838230719 51.3793 88.9411
11 0.000499095 0.991970541 1990.01 4054.49
12 0.014117706 0.89666719 137.404 283.169
13 -0.024466331 0.796311149 171.77 324.231
14 0.001172524 0.985524043 322.255 644.278
15 0.03002549 0.592754532 483.867 1031.77
16 1 -9.77473 -1.3937
17 -0.017840578 0.809155743 250.499 480.201
18 -0.012054001 0.82145941 656.21 1272.34
19 -0.336048272 9.66E-10 65174.8 65177
20 0.016112443 0.859016204 174.212 361.219

Total number of rows: 22575

Table truncated, full table size 997 Kbytes.




Supplementary file Size Download File type/resource
GSM182342.txt.gz 5.2 Mb (ftp)(http) TXT

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