|
Status |
Public on Apr 01, 2008 |
Title |
dye-swap experiment between shoot submarged in water for 24 hours and non-treated shoot 3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
10 days old shoot10
|
Organism |
Oryza sativa Japonica Group |
Characteristics |
cultivar: Nipponbare Tissue: shoot stage: LP.03 three leaves visible age: 10 days after sowing
|
Growth protocol |
growth: hydroponic culture growth enviroment: growth chamber study Growth substrates: 1/5000 diluted Hyponex (10-3-3) solution light condition: 16hrs day length temperature: 28 degreeC
|
Extracted molecule |
total RNA |
Extraction protocol |
Separation technique: Trimming Extraction source: Frozen Kit: RNeasy Midi Kit Manufacturer: QIAGEN Manual: QIAGEN
|
Label |
Cy3
|
Label protocol |
Kit: Low RNA Input Linear Amplification kit Manufacturer: Agilent Technologies Manual: Low RNA Input Linear Amplification kit standard protocol
|
|
|
Channel 2 |
Source name |
shoot submarged in water for 24 hours2
|
Organism |
Oryza sativa Japonica Group |
Characteristics |
cultivar: Nipponbare Tissue: shoot stage: LP.03 three leaves visible age: 10 days after sowing
|
Treatment protocol |
aboitic factor: flood water stress factor: flood stress factor: 24 hour
|
Growth protocol |
growth: hydroponic culture growth enviroment: growth chamber study Growth substrates: 1/5000 diluted Hyponex (10-3-3) solution light condition: 16hrs day length temperature: 28 degreeC
|
Extracted molecule |
total RNA |
Extraction protocol |
Separation technique: Trimming Extraction source: Frozen Kit: RNeasy Midi Kit Manufacturer: QIAGEN Manual: QIAGEN
|
Label |
Cy5
|
Label protocol |
Kit: Low RNA Input Linear Amplification kit Manufacturer: Agilent Technologies Manual: Low RNA Input Linear Amplification kit standard protocol
|
|
|
|
Hybridization protocol |
hybridization_Kit: in situ hybridization plus hybridization_Manufacturer: Agilent Technologies hybridization_Manual: Agilent Technologies
|
Scan protocol |
Scanner: Agilent DNA microarray Scaner Manufacturer: Agilent Technologies Software: Scanner Control software Manufacturer: Agilent Technologies Manual: Agilent Technologies
|
Description |
10 days old shoot10' is control sample, and is same as sample of sample name shoot_self10. 'shoot submarged in water for 24 hours2' is target sample.
|
Data processing |
Software: Feature Extraction ver 8.1.1.1 Manufacturer: Agilent Technologies Manual: Agilent Technologies
|
|
|
Submission date |
Apr 16, 2007 |
Last update date |
Apr 16, 2007 |
Contact name |
Kouji Satoh |
Organization name |
NARO/HARC
|
Street address |
Hitsujigaoka 1
|
City |
Sapporo |
State/province |
Hokkaido |
ZIP/Postal code |
062-8555 |
Country |
Japan |
|
|
Platform ID |
GPL892 |
Series (1) |
GSE7532 |
Differentially expressed genes under different stress conditions in rice |
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