|
| Status |
Public on Apr 26, 2007 |
| Title |
Human 251 (Hs3) Anterior Inferior Temporal Cortex |
| Sample type |
RNA |
| |
|
| Source name |
Human brain anterior inferior temporal cortex from the left hemisphere
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: Male
Age: 71 years
Tissue: Anterior inferior temporal cortex
Origin: Autopsy (PMI = 3 hours)
|
| Treatment protocol |
Tissue samples (1-5 g) were immediately frozen after dissection in liquid nitrogen and kept at –80°C until used for RNA extraction. Around 100 mg of each tissue sample were then separated on ice, placed on a tube containing 1 ml of Trizol, and homogenized with a 7-mm polytron for 2 min.
|
| Growth protocol |
Brain tissue samples were dissected during autopsy or surgical procedures.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Around 100 mg of each tissue sample were isolated on ice, placed on a tube containing 1 ml of Trizol, and homogenized with a 7-mm polytron for 2 min. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, hybridizations were performed on Affymetrix HG_U95Av2 GeneChips for 16 hr at 50C at a final cRNA concentration of 0.06 ug/ul. After hybridization, GeneChips were washed in the Affymetrix Fluidics Station and stained manually following the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner with default parameters. Two scans were performed for each array.
|
| Description |
Gene expression data from brain middle frontal gyrus cortex of human 251 (Hs3).
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
|
| |
|
| Submission date |
Apr 18, 2007 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Mario Cáceres |
| E-mail(s) |
mario.caceres@crg.es
|
| Phone |
34-93-3160233
|
| Fax |
34-93-3160099
|
| Organization name |
Center for Genomic Regulation
|
| Department |
Genes and Disease Program
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE7540 |
Gene expression analysis of the human and chimpanzee brain |
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