|
| Status |
Public on Sep 21, 2015 |
| Title |
Fibroblasts_ATS_P1 |
| Sample type |
RNA |
| |
|
| Source name |
Skin fibroblasts
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: skin fibroblast
disease state: arterial tortuosity syndrome
|
| Growth protocol |
Fibroblast cells were cultured in Eagle's minimum essential (MEM) supplemented with 10% FBS, 100 mg/mL streptomycin, 100 U/mL penicillin and incubated at 37° C in a humidified chamber with 5% CO2. Cells were harvested once they reached confluence by treating with trypsin (0.05% trypsin with 0.25% EDTA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from skin fibroblasts of 3 ATS patients and 3 unrelated controls using the Qiagen RNeasy kit according to manufactures’ instructions (Qiagen, Hilden, Germany).
|
| Label |
Biotin
|
| Label protocol |
250 ng of total RNA was labeled using the Flash Tag Biotin RNA Labeling Kit for Affymetrix® GeneChip® miRNA Arrays according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Labeled RNA was hybridized for 16 hr at 45° C on GeneChip miRNA 3.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G (Affimetrix, SantanClara, CA).
|
| Data processing |
The data were analyzed with Expression Console™ Software using Affymetrix default analysis settings and Robust Multichip Analysis (RMA). Subsequently analyses for differentially expressed genes cathegorization were performed with Partek® Genomics Suite software version 6.6 (Partek Inc., St. Louis, MO).
|
| |
|
| Submission date |
Jul 21, 2015 |
| Last update date |
May 18, 2022 |
| Contact name |
Nicola Chiarelli |
| E-mail(s) |
nicola.chiarelli@unibs.it
|
| Organization name |
University of Brescia
|
| Department |
Molecular and Translational Medicine
|
| Lab |
Biology and Genetics
|
| Street address |
Viale Europa 11
|
| City |
Brescia |
| State/province |
Lombardy |
| ZIP/Postal code |
25123 |
| Country |
Italy |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE71172 |
microRNA expression profiles from three arterial tortuosity syndrome (ATS) patients' skin fibroblasts with recessive SLC2A10 mutations [miRNA] |
|
