|
Status |
Public on Mar 12, 2016 |
Title |
RNA-Seq of ume6 deletion (with Chd1-Ume6) |
Sample type |
SRA |
|
|
Source name |
RNA-Seq of ume6 deletion (with Chd1-Ume6)
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain background: W303 genotype/variation: ume6 deletion (with Chd1-Ume6) isolate number: isolate 1 presence of chd1-ume6 fusion: yes
|
Growth protocol |
cells were grown to OD600 0.4 to 0.6
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with hot acid phenol, treated with Dnase I, and depleted of rRNA Strand-specific libraries were prepared according to dUTP method and TruSeq (Illumina) Sample Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
Basecalling was performed by Illumina CASAVA software package Reads were mapped to S. cerevisiae genome (Saccharomyces_cerevisiae.EF4.65.dna.toplevel.fa) appended with ERCC Spike-in Control reads (https://tools.lifetechnologies.com/content/sfs/manuals/ERCC92.zip) using TopHat2 Mapped reads were filtered using SAMtools flags -f 3 -F 256 Differential transcript analysis was performed using CuffDiff (cufflinks 2.2.1) with a maskfile for rRNA and tRNA Independent isolates were merged Genome_build: Saccharomyces_cerevisia.EF4.65 Supplementary_files_format_and_content: Normalized FPKM Values of all Genes for Indicated Strains. The existance of a Ume6 binding motif (WNGGCGGCWW) within 500 base pairs of the associated gene is indicated by Has_Motif = yes.
|
|
|
Submission date |
Sep 01, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Jeffrey N McKnight |
E-mail(s) |
jmcknig2@uoregon.edu
|
Organization name |
University of Oregon
|
Department |
Institute of Molecular Biology
|
Lab |
McKnight Lab
|
Street address |
1229 University of Oregon, 1318 Franklin Blvd, Rm 273 Onyx Bridge
|
City |
Eugene |
State/province |
OR |
ZIP/Postal code |
97408 |
Country |
USA |
|
|
Platform ID |
GPL9134 |
Series (2) |
GSE72571 |
Sequence-Targeted Nucleosome Sliding in vivo - Transcription Profiling |
GSE72572 |
Sequence-Targeted Nucleosome Sliding in vivo |
|
Relations |
BioSample |
SAMN04024971 |
SRA |
SRX1176436 |