|
| Status |
Public on Aug 31, 2017 |
| Title |
201154 - Negative |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: whole blood
sample type: reference
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
We extracted DNA from the PAXgene (Agilent, Inc.) tube; whole blood collected from the volenteers stored in Paxgen tube as per the vendor's recommandation.
|
| Label |
Cy3
|
| Label protocol |
Total Genomic DNA (5µg) was used as starting material, using the SureTag Complete DNA Labeling Kit (Agilent Technologies). Labeled DNA was quantified using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific Inc, Waltham, MA, USA). The IP (sample of interest) samples were labeled with Cy5 and the WEC (reference) were labeled with Cy3.
|
| |
|
| Channel 2 |
| Source name |
Whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: whole blood
sample type: IP
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
We extracted DNA from the PAXgene (Agilent, Inc.) tube; whole blood collected from the volenteers stored in Paxgen tube as per the vendor's recommandation.
|
| Label |
Cy5
|
| Label protocol |
Total Genomic DNA (5µg) was used as starting material, using the SureTag Complete DNA Labeling Kit (Agilent Technologies). Labeled DNA was quantified using a NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific Inc, Waltham, MA, USA). The IP (sample of interest) samples were labeled with Cy5 and the WEC (reference) were labeled with Cy3.
|
| |
|
| |
| Hybridization protocol |
we loaded 2.5 to 6µg DNA to the array
|
| Scan protocol |
Scanned on a Agilent Technologies SureScan Microarray Scanner G2600D
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version11.5.1.1). Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Dec 29, 2015 |
| Last update date |
Aug 31, 2017 |
| Contact name |
Aarti Gautam |
| E-mail(s) |
aarti.gautam.civ@health.mil
|
| Phone |
301-619-7683
|
| Organization name |
WRAIR
|
| Department |
Medical Readiness Systems Biology
|
| Street address |
503 Robert Grant Avenue
|
| City |
Silver Spring |
| State/province |
MD |
| ZIP/Postal code |
20910 |
| Country |
USA |
| |
|
| Platform ID |
GPL19930 |
| Series (1) |
| GSE76401 |
Epigenomic study of Post-traumatic Stress Disorder among OIF/OEF veterans |
|
