|
| Status |
Public on Mar 03, 2016 |
| Title |
NCvsNN-Infantile Hemangioma-3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from pooled infantile hemangioma
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
|
| Treatment protocol |
Paired tissues of infantile hemangioma and peri-tumor tissues from every patient were quick-frozen by liquid nitrogen as soon as possible and then were moved to -80℃ condition for long-term storage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy5
|
| Label protocol |
Labeled RNAs were heat denatured and hybridized to Agilent lncRNA microarray, according to manufacturer's protocol.
|
| |
|
| Channel 2 |
| Source name |
Total RNA from pooled adjacent nontumor skin
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
|
| Treatment protocol |
Paired tissues of infantile hemangioma and peri-tumor tissues from every patient were quick-frozen by liquid nitrogen as soon as possible and then were moved to -80℃ condition for long-term storage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
Labeled RNAs were heat denatured and hybridized to Agilent lncRNA microarray, according to manufacturer's protocol.
|
| |
|
| |
| Hybridization protocol |
According to the Agilent lncRNA Microarray Protocol (Agilent Technologies).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
Images were quantified using Agilent Feature Extraction(v10.7).
|
| Description |
Biological replicate 3 of 3.Infantile hemangioma
|
| Data processing |
LOWESS normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal.Feature Extraction v10.7.3.1 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Mar 02, 2016 |
| Last update date |
Mar 03, 2016 |
| Contact name |
Xiaowen Liu |
| E-mail(s) |
qqwen15@126.com
|
| Organization name |
Shandong Provincial Hospital affiliated to Shandong University
|
| Street address |
Jingwu Road 324
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250021 |
| Country |
China |
| |
|
| Platform ID |
GPL20115 |
| Series (1) |
| GSE78811 |
lncRNA expression profile identified by microarray in infantile hemangioma |
|
