|
| Status |
Public on Nov 09, 2007 |
| Title |
Ewing Sarcoma Tumour Sample # 22 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Genomic DNA from ES sample # 22 labeled with Cyanine-5 (red).
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Female
Site: Rib
Stage: M (metastatic)
Genetic: UNST (unstable)
Gain_Chr8: No
Status: DOD (dead of disease)
Follow-up: 93
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using Quiagen's Tissue Kit following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
2 µg of genomic DNA were primed with 20 µl of 2.5x Random Primers at 95°C for 5 min and then labeled with 5µl of 10x dUTP mix, 3 µl of Cy3 or Cy5 dUTP (1.0mM) and 1 µl of Exp-Klenow at 37ºC for 3 hours.
|
| |
|
| Channel 2 |
| Source name |
Genomic DNA from pooled pheripheral blood of ten healthy female donors
|
| Organism |
Homo sapiens |
| Characteristics |
control; pooled peripheral blood of ten healthy female donors
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using Quiagen's Tissue Kit following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
2 µg of genomic DNA were primed with 20 µl of 2.5x Random Primers at 95°C for 5 min and then labeled with 5µl of 10x dUTP mix, 3 µl of Cy3 or Cy5 dUTP (1.0mM) and 1 µl of Exp-Klenow at 37ºC for 3 hours.
|
| |
|
| |
| Hybridization protocol |
Human cot-1 DNA, Agilent 10x blocking agent and Agilent 2x hybridization buffer were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
Images were quantified using Agilent Feature Extraction Software (version v:8.1).
|
| Description |
Primary Ewing Sarcoma Tumour Sample # 22
|
| Data processing |
LINEAR normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Jul 06, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Juan C Cigudosa |
| E-mail(s) |
jccigudosa@cnio.es
|
| Organization name |
Centro Nacional de Investigaciones Oncologicas - CNIO
|
| Department |
Human Cancer Genetics
|
| Lab |
Molecular Cytogenetics
|
| Street address |
C/ Melchor Fernández Almagro, 3
|
| City |
Madrid |
| ZIP/Postal code |
E-28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL2879 |
| Series (1) |
| GSE8398 |
CGH profiling of 26 Ewing Sarcoma tumour samples |
|
