|
| Status |
Public on Aug 29, 2008 |
| Title |
shLoxl2_2 CY3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
shLoxl2_2 CY3
|
| Organism |
Mus musculus |
| Characteristics |
Haca cells shLoxl2 labelled with cy3 dye
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNAesy Extraction Kit (QIAGen).
|
| Label |
cy3
|
| Label protocol |
Labelling and hybridization were performed using Low RNA Linear Amplification Kit and In Situ Hybridization Kit Plus (Agilent), respectively, following manufacturer’s protocol.
|
| |
|
| Channel 2 |
| Source name |
control cell line shGFP CY5
|
| Organism |
Mus musculus |
| Characteristics |
Haca cells shGFP labelled with cy5 dye
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNAesy Extraction Kit (QIAGen).
|
| Label |
CY5
|
| Label protocol |
Labelling and hybridization were performed using Low RNA Linear Amplification Kit and In Situ Hybridization Kit Plus (Agilent), respectively, following manufacturer’s protocol.
|
| |
|
| |
| Hybridization protocol |
Labelling and hybridization were performed using Low RNA Linear Amplification Kit and In Situ Hybridization Kit Plus (Agilent), respectively, following manufacturer’s protocol.
|
| Scan protocol |
After hybridization and washing, slides were scanned in an Axon GenePix Scanner (Axon Instruments, Inc., Union City, CA) and analyzed using Feature Extraction Software 6.1.1 (Agilent).
|
| Description |
Labelling and hybridization were performed using Low RNA Linear Amplification Kit and In Situ Hybridization Kit Plus (Agilent), respectively, following manufacturer’s protocol.
|
| Data processing |
analyzed using Feature Extraction Software 6.1.1 (Agilent).
|
| |
|
| Submission date |
Jul 24, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Gema Moreno-Bueno |
| E-mail(s) |
gmoreno@iib.uam.es
|
| Phone |
+34914978974
|
| Organization name |
IIB
|
| Department |
Biochemistry
|
| Lab |
1.13
|
| Street address |
Arturo Duperier
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL2881 |
| Series (1) |
| GSE8568 |
EXPRESSION PROFILING IN shlOXL2 AND shSNAIL IN HACA CELLS |
|
