|
Status |
Public on Aug 03, 2007 |
Title |
Biopsy of a colorectal adenoma from patient #14 |
Sample type |
RNA |
|
|
Source name |
Human colorectal adenoma
|
Organism |
Homo sapiens |
Characteristics |
Tissue: adenoma Location: ascending colon Size: 1.5 cm Patient ID: SD CEL Filename: 040205_SD_po (vi_po_17).CEL, where "nm" is for normal mucosa (colorectal) and "po" is for polyp (colorectal)"
|
Extracted molecule |
total RNA |
Extraction protocol |
For each polyp, three biopsies of normal mucosa were collected from the same colon segment (> 2 cm from the site of the polyp). Immediately after removal, a small sample of epithelial tissue (5-15 mg) was cut from the tip of each polyp, leaving the underlying muscularis mucosae intact. Total RNA was extracted (RNeasy Mini kit, Qiagen) from homogenized tissue samples (5- to 15-mg), and its integrity was verified by capillary gel electrophoresis (Bio Analyzer, Agilent Technologies).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on HG-133 Plus2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Gene expression data from colorectal adenoma.
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
|
|
|
Submission date |
Aug 02, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Giancarlo Marra |
E-mail(s) |
marra@imcr.uzh.ch
|
Phone |
0041 44 6353472
|
Fax |
0041 44 6353484
|
Organization name |
University of Zurich
|
Department |
Molecular Cancer Research
|
Street address |
Winterthurerstrasse 190
|
City |
Zurich |
ZIP/Postal code |
8057 |
Country |
Switzerland |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE8671 |
Transcriptome profile of human colorectal adenomas. |
|
Relations |
Reanalyzed by |
GSE64985 |
Reanalyzed by |
GSE119087 |