|
| Status |
Public on Aug 21, 2007 |
| Title |
Rice_BPH_rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Rice leaves, infested with brown planthopper (BPH) for 24 hours.
|
| Organism |
Oryza sativa |
| Characteristics |
Cultivar: a Japonica cultivar "Hinohikari"
Leaf-stage : 5-leaf-stage
Tissue: leaf
|
| Treatment protocol |
Rice plants were encased in transparent plastic cylinders (70 cm x 15 cm) and 20 adult brown planthopper (Nilaparvata lugens Stål) males per plant were released in the cylinder for 24 h.
|
| Growth protocol |
Rice plants (Oryza sativa L. cv. Hinohikari) were grown from seed planted in plastic pots containing commercial soil (Hinokunishodo; Japan agricultural Co., Tokyo, Japan). The pots were then submerged in water inside a container and was kept in a glasshouse at 25 ± 1 ℃, 60-80 % RH under natural sunlight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Kit: Trizol
Manufacturer: Invitrogen
|
| Label |
Cy5
|
| Label protocol |
Kit: Low input RNA linear amplification kit
Manufacturer: Agilent Technologies
|
| |
|
| Channel 2 |
| Source name |
Rice leaves, mock-treated.
|
| Organism |
Oryza sativa |
| Characteristics |
Cultivar: a Japonica cultivar "Hinohikari"
Leaf-stage : 5-leaf-stage
Tissue: leaf
|
| Treatment protocol |
Rice plants were encased in transparent plastic cylinders (70 cm x 15 cm) without insects for 24 h.
|
| Growth protocol |
Rice plants (Oryza sativa L. cv. Hinohikari) were grown from seed planted in plastic pots containing commercial soil (Hinokunishodo; Japan agricultural Co., Tokyo, Japan). The pots were then submerged in water inside a container and was kept in a glasshouse at 25 ± 1 ℃, 60-80 % RH under natural sunlight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Kit: Trizol
Manufacturer: Invitrogen
|
| Label |
Cy3
|
| Label protocol |
Kit: Low input RNA linear amplification kit
Manufacturer: Agilent Technologies
|
| |
|
| |
| Hybridization protocol |
Kit: in situ hybridization kit plus
Manufacturer: Agilent Technologies
|
| Scan protocol |
Scanner: Agilent DNA microarray scanner
Manufacturer: Agilent Technologies
|
| Description |
Rice plants at the 5-leaf-stage were encased in transparent plastic cylinders (70 cm x 15 cm) and 20 adult brown planthopper (Nilaparvata lugens Stål) males per plant were released in the cylinder. Control plants were put in transparent plastic cylinders without insects. After 24 h, all plants were removed from the cylinders and all insects were removed from the rice plants. Fourth and fifth leaf blades infested with or without insects were collected and frozen in liquid nitrogen. Leaves were collected from three rice plants with or without insects. Then we pooled leaves from multiple plants as the source of RNA. The frozen stored leaves were ground in liquid nitrogen thoroughly with a pestle. Total RNA was extracted from the powder of leaves using Trizol.
|
| Data processing |
The TIFF image was used to extract raw and processed data using Agilent’s Feature Extraction software ver 8.1.1.1. Raw result of data processing with Agilent’s Feature Extraction software was provided as Supplementary text file.
|
| |
|
| Submission date |
Aug 16, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Masaru Satoh |
| E-mail(s) |
msatou@affrc.go.jp
|
| Phone |
+81-96-242-7731
|
| Fax |
+81-96-249-1002
|
| Organization name |
National Agricultural Research Center for Kyushu Okinawa Region
|
| Lab |
Research Team for Insect Pest and Nematode Management
|
| Street address |
2421, Suya
|
| City |
Koshi |
| State/province |
Kumamoto |
| ZIP/Postal code |
861-1192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE8811 |
Rice plants infested with planthoppers |
|
