|
| Status |
Public on Sep 17, 2007 |
| Title |
MLP-29 KrasG12D Clone2 |
| Sample type |
RNA |
| |
|
| Source name |
MLP-29, knock-in of G12D mutation in one Kras allele, Clone2
|
| Organism |
Mus musculus |
| Characteristics |
Liver progenitor cell line derived from E13 mouse embryo
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction was performed using Trizol plus RNA purification kit (Invitrogen). RNA qualitative and quantitative assessment was performed using the Bioanalyzer 2100 (Agilent Tecnologies).
|
| Label |
biotin
|
| Label protocol |
Five hundred ng of total RNA was reverse transcribed and amplified overnight with T7 RNA polymerase and labeled with biotin following the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
0.85 microgram of biotin-labeled cRNA was hybridized to Illumina Mouse Ref-8 BeadChips at 55 °C overnight. BeadChips were incubated with Cy3 streptavidin and washed according to the manufacturer’s protocol.
|
| Scan protocol |
The hybridized BeadChips were scanned by Illumina BeadScan confocal scanner
|
| Description |
Oncogenic G12D mutation inserted by AAV-driven homologous recombination at the Kras gene in one allele.
|
| Data processing |
Illumina BeadStudio version 1.5.1.3
|
| |
|
| Submission date |
Aug 22, 2007 |
| Last update date |
Mar 21, 2008 |
| Contact name |
Enzo Medico |
| E-mail(s) |
enzo.medico@ircc.it
|
| Phone |
+39-011-9933234
|
| Organization name |
Candiolo Cancer Institute, University of Torino
|
| Department |
Oncology
|
| Lab |
Laboratory of Oncogenomics
|
| Street address |
Strada Prov. 142, km 3,95
|
| City |
Candiolo |
| State/province |
TO |
| ZIP/Postal code |
10060 |
| Country |
Italy |
| |
|
| Platform ID |
GPL4865 |
| Series (1) |
| GSE8711 |
Knock-in of Kras G12D in mouse MLP-29 cells |
|
