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Status |
Public on Jan 01, 2008 |
Title |
MALT sample # 79 |
Sample type |
genomic |
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|
Channel 1 |
Source name |
Genomic DNA from MALT sample # 79 labeled with Cyanine-5 (red)
|
Organism |
Homo sapiens |
Characteristics |
MALT
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Total DNA extracted using Quiagen's Tissue Kit following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
2 µg of genomic DNA were primed with 20 µl of 2.5x Random Primers at 95°C for 5 min and then labeled with 5µl of 10x dUTP mix, 3 µl of Cy3 or Cy5 dUTP (1.0mM) and 1 µl of Exp-Klenow at 37ºC for 3 hours.
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|
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Channel 2 |
Source name |
Genomic DNA from pooled pheripheral blood of ten healthy female donors
|
Organism |
Homo sapiens |
Characteristics |
no additional information
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Total DNA extracted using Quiagen's Tissue Kit following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
2 µg of genomic DNA were primed with 20 µl of 2.5x Random Primers at 95°C for 5 min and then labeled with 5µl of 10x dUTP mix, 3 µl of Cy3 or Cy5 dUTP (1.0mM) and 1 µl of Exp-Klenow at 37ºC for 3 hours.
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|
|
|
Hybridization protocol |
Human cot-1 DNA, Agilent 10x blocking agent and Agilent 2x hybridization buffer were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
Scan protocol |
Scanned on an Agilent G2565AA scanner. Images were quantified using Agilent Feature Extraction Software (version v:8.1).
|
Description |
none
|
Data processing |
LINEAR normalized, background subtracted data obtained from log2 of processed Red signal/processed Green signal. Agilent software was used.
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|
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Submission date |
Aug 30, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Juan C Cigudosa |
E-mail(s) |
jccigudosa@cnio.es
|
Organization name |
Centro Nacional de Investigaciones Oncologicas - CNIO
|
Department |
Human Cancer Genetics
|
Lab |
Molecular Cytogenetics
|
Street address |
C/ Melchor Fernández Almagro, 3
|
City |
Madrid |
ZIP/Postal code |
E-28029 |
Country |
Spain |
|
|
Platform ID |
GPL2879 |
Series (1) |
GSE8918 |
CGH profiling of 87 indolent non-hodgkin’s lymphoma (NHL) |
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