|
| Status |
Public on Nov 01, 2007 |
| Title |
12m x mgb1 24h-1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mock infection
|
| Organism |
Oryza sativa |
| Characteristics |
cultivar : IRBL12-M
Age : 14 days
Tissue : Leaf
|
| Treatment protocol |
Fourteen days old rice plants were sprayed with conidia-free water and inoculated in a dew chamber at 25°C for 16 h then transferred to a greenhouse. Total RNAs were isolated from fourth leaves at 24hpi.
|
| Growth protocol |
Ten rice seeds (Oryza sativa L. cv. IRBL12-M) were sown in a pot that has been filled with a commercial soil mixture (Bonsol type 1; Sumitomo Chemical, Osaka, Japan). The pot was then submerged in water inside a container and was kept in a greenhouse at 28 degreeC under natural sunlight for 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit : RNeasy mini kit
Manufacture : Qiagen
|
| Label |
Cy3
|
| Label protocol |
kit : Low input RNA linear amplification kit
Manufacture : Agilent Technologies
|
| |
|
| Channel 2 |
| Source name |
mgb1 infection
|
| Organism |
Oryza sativa |
| Characteristics |
cultivar : IRBL12-M
Age : 14 days
Tissue : Leaf
mgb1 : A null mutant of the heterotrimeric G-protein b-subunit, which is impaired in the surface recognition and consequently in the initiation of appressorium formation.
|
| Treatment protocol |
Fourteen days old rice plants were sprayed with mgb1 conidia suspension (100,000/mL) and inoculated in a dew chamber at 25°C for 16 h then transferred to a greenhouse. Total RNAs were isolated from fourth leaves at 24hpi.
|
| Growth protocol |
Ten rice seeds (Oryza sativa L. cv. IRBL12-M) were sown in a pot that has been filled with a commercial soil mixture (Bonsol type 1; Sumitomo Chemical, Osaka, Japan). The pot was then submerged in water inside a container and was kept in a greenhouse at 28 degreeC under natural sunlight for 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
kit : RNeasy mini kit
Manufacture : Qiagen
|
| Label |
Cy5
|
| Label protocol |
kit : Low input RNA linear amplification kit
Manufacture : Agilent Technologies
|
| |
|
| |
| Hybridization protocol |
Kit : in situ hybridization kit plus
Manufacture : Agilent Technologies
|
| Scan protocol |
Scanner : Agilent DNA microarray scanner
Manufacture: Agilent Technologies
|
| Description |
Keyword : Oryza sativa
Keyword : Magnaporthe grisea
Mgb1 is a mutant of the Magnaporthe grisea, which is impaired in the surface recognition and consequently in the initiation of appressorium formation.
|
| Data processing |
Feature Extraction ver 9.1 (Agilent Technologies)
|
| |
|
| Submission date |
Oct 28, 2007 |
| Last update date |
Oct 30, 2007 |
| Contact name |
Tomoaki Kato |
| E-mail(s) |
tomkato@nias.affrc.go.jp
|
| Organization name |
National Institute of Agrobiological Sciences
|
| Department |
Division of Plant Sciences
|
| Lab |
Plant-Microbe Interactions Research Unit
|
| Street address |
2-1-2 Kannondai
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8602 |
| Country |
Japan |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE9450 |
Rice plants infected by Rice blast fungus |
|
| Data table header descriptions |
| ID_REF |
|
| VALUE |
log10 [normalized Ch2 / normalized Ch1] |
| PVALUE |
Significance level of the value computed for a feature |
| NORM_SIG_CH1 |
CH1: Dye-normalized signal intensity of CH1 |
| NORM_SIG_CH2 |
CH2: Dye-normalized signal intensity of CH2 |
| MeanSignal_CH1 |
Raw mean CH1 signal of feature |
| MeanSignal_CH2 |
Raw mean CH2 signal of feature |
| BGMeanSignal_CH1 |
CH1: Mean local background CH1 signal |
| BGMeanSignal_CH2 |
CH1: Mean local background CH2 signal |
