|
Status |
Public on Nov 01, 2007 |
Title |
LTH x mgb1 24h-3 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Mock infection
|
Organism |
Oryza sativa |
Characteristics |
cultivar : LTH Age : 14 days Tissue : Leaf
|
Treatment protocol |
Fourteen days old rice plants were sprayed with conidia-free water and inoculated in a dew chamber at 25 degreeC for 16 h then transferred to a greenhouse. Total RNAs were isolated from fourth leaves at 24hpi.
|
Growth protocol |
Ten rice seeds (Oryza sativa L. cv. LTH) were sown in a pot that has been filled with a commercial soil mixture (Bonsol type 1; Sumitomo Chemical, Osaka, Japan). The pot was then submerged in water inside a container and was kept in a greenhouse at 28 degreeC under natural sunlight for 14 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
kit : RNeasy mini kit Manufacture : Qiagen
|
Label |
Cy3
|
Label protocol |
kit : Low input RNA linear amplification kit Manufacture : Agilent Technologies
|
|
|
Channel 2 |
Source name |
mgb1 infection
|
Organism |
Oryza sativa |
Characteristics |
cultivar : LTH Age : 14 days Tissue : Leaf mgb1 : A null mutant of the heterotrimeric G-protein b-subunit, which is impaired in the surface recognition and consequently in the initiation of appressorium formation.
|
Treatment protocol |
Fourteen days old rice plants were sprayed with mgb1 conidia suspension (100,000/mL) and inoculated in a dew chamber at 25 degreeC for 16 h then transferred to a greenhouse. Total RNAs were isolated from fourth leaves at 24hpi.
|
Growth protocol |
Ten rice seeds (Oryza sativa L. cv. LTH) were sown in a pot that has been filled with a commercial soil mixture (Bonsol type 1; Sumitomo Chemical, Osaka, Japan). The pot was then submerged in water inside a container and was kept in a greenhouse at 28 degreeC under natural sunlight for 14 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
kit : RNeasy mini kit Manufacture : Qiagen
|
Label |
Cy5
|
Label protocol |
kit : Low input RNA linear amplification kit Manufacture : Agilent Technologies
|
|
|
|
Hybridization protocol |
Kit : in situ hybridization kit plus Manufacture : Agilent Technologies
|
Scan protocol |
Scanner : Agilent DNA microarray scanner Manufacture: Agilent Technologies
|
Description |
Keyword : Oryza sativa Keyword : Magnaporthe grisea Mgb1 is a mutant of the Magnaporthe grisea, which is impaired in the surface recognition and consequently in the initiation of appressorium formation.
|
Data processing |
Feature Extraction ver 9.1 (Agilent Technologies)
|
|
|
Submission date |
Oct 29, 2007 |
Last update date |
Oct 30, 2007 |
Contact name |
Tomoaki Kato |
E-mail(s) |
tomkato@nias.affrc.go.jp
|
Organization name |
National Institute of Agrobiological Sciences
|
Department |
Division of Plant Sciences
|
Lab |
Plant-Microbe Interactions Research Unit
|
Street address |
2-1-2 Kannondai
|
City |
Tsukuba |
State/province |
Ibaraki |
ZIP/Postal code |
305-8602 |
Country |
Japan |
|
|
Platform ID |
GPL892 |
Series (1) |
GSE9450 |
Rice plants infected by Rice blast fungus |
|
Data table header descriptions |
ID_REF |
|
VALUE |
log10 [normalized Ch2 / normalized Ch1] |
PVALUE |
Significance level of the value computed for a feature |
NORM_SIG_CH1 |
Dye-normalized signal intensity of CH1 |
NORM_SIG_CH2 |
Dye-normalized signal intensity of CH2 |
MeanSignal_CH1 |
Raw mean CH1 signal of feature |
MeanSignal_CH2 |
Raw mean CH2 signal of feature |
BGMeanSignal_CH1 |
Mean local background CH1 signal |
BGMeanSignal_CH2 |
Mean local background CH2 signal |