|
Status |
Public on Dec 31, 2020 |
Title |
shoot of OsPI1 knockdown rice grown under phosphorus sufficiency, replication 1. |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Shoot of wild type (cv. Kitaake), replication 1
|
Organism |
Oryza sativa |
Characteristics |
Shoot samples from control plants grown under phosphorus sufficiency.
|
Treatment protocol |
Phosphorus sufficiency (1 ppm)
|
Growth protocol |
Plants were grown in nutrient solutions with phosphorus. Shoot samples were harvested 9 d after the treatment.
|
Extracted molecule |
total RNA |
Extraction protocol |
Plant RNA Isolation Mini Kit (Agilent Technologies) was used according to manufacturers protocol.
|
Label |
Cy3
|
Label protocol |
Standard Protocol of Agilent Low RNA Input Linear Amplification Kit
|
|
|
Channel 2 |
Source name |
Shoot of ospi1-2 (OsPI1 knockdown line), replication 1
|
Organism |
Oryza sativa |
Characteristics |
Shoot samples from ospi1-2 plants grown under phosphorus sufficiency.
|
Treatment protocol |
Phosphorus sufficiency (1 ppm)
|
Growth protocol |
Plants were grown in nutrient solutions with phosphorus. Shoot samples were harvested 9 d after the treatment.
|
Extracted molecule |
total RNA |
Extraction protocol |
Plant RNA Isolation Mini Kit (Agilent Technologies) was used according to manufacturers protocol.
|
Label |
Cy5
|
Label protocol |
Standard Protocol of Agilent Low RNA Input Linear Amplification Kit
|
|
|
|
Hybridization protocol |
Standard protocol of In Situ Hybridization Kit Plus from Agilent Technologies.
|
Scan protocol |
Standard protocol of Agilent DNA Microarray Scanner
|
Description |
Keyword = rice Keyword = Phosphorus deficiency response Keyword = knockdown line of OsPI1 (Oryza sativa Phosphate-limitation Inducible gene 1)
|
Data processing |
Feature Extraction software Version 8.5.1.1 from Agilent Technologies
|
|
|
Submission date |
Jan 31, 2008 |
Last update date |
Dec 31, 2020 |
Contact name |
Jun Wasaki |
E-mail(s) |
junw@hiroshima-u.ac.jp
|
Organization name |
Hiroshima University
|
Department |
Graduate School of Biosphere Science
|
Street address |
Kagamiyama 1-7-1
|
City |
Higashi-Hiroshima |
State/province |
Hiroshima |
ZIP/Postal code |
739-8521 |
Country |
Japan |
|
|
Platform ID |
GPL892 |
Series (1) |
GSE10335 |
Transcriptional profiling of shoot of OsPI1 knockdown rice |
|