|
| Status |
Public on Dec 31, 2020 |
| Title |
shoot of OsPI1 knockdown rice grown under phosphorus sufficiency, replication 2. |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Shoot of ospi1-2 (OsPI1 knockdown line), replication 2
|
| Organism |
Oryza sativa |
| Characteristics |
Shoot samples from ospi1-2 plants grown under phosphorus sufficiency.
|
| Treatment protocol |
Phosphorus sufficiency (1 ppm)
|
| Growth protocol |
Plants were grown in nutrient solutions with phosphorus. Shoot samples were harvested 9 d after the treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plant RNA Isolation Mini Kit (Agilent Technologies) was used according to manufacturers protocol.
|
| Label |
Cy3
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Linear Amplification Kit
|
| |
|
| Channel 2 |
| Source name |
Shoot of wild type (cv. Kitaake), replication 2
|
| Organism |
Oryza sativa |
| Characteristics |
Shoot samples from control plants grown under phosphorus sufficiency.
|
| Treatment protocol |
Phosphorus sufficiency (1 ppm)
|
| Growth protocol |
Plants were grown in nutrient solutions with phosphorus. Shoot samples were harvested 9 d after the treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Plant RNA Isolation Mini Kit (Agilent Technologies) was used according to manufacturers protocol.
|
| Label |
Cy5
|
| Label protocol |
Standard Protocol of Agilent Low RNA Input Linear Amplification Kit
|
| |
|
| |
| Hybridization protocol |
Standard protocol of In Situ Hybridization Kit Plus from Agilent Technologies.
|
| Scan protocol |
Standard protocol of Agilent DNA Microarray Scanner
|
| Description |
Keyword = rice
Keyword = Phosphorus deficiency response
Keyword = knockdown line of OsPI1 (Oryza sativa Phosphate-limitation Inducible gene 1)
|
| Data processing |
Feature Extraction software Version 8.5.1.1 from Agilent Technologies
|
| |
|
| Submission date |
Jan 31, 2008 |
| Last update date |
Dec 31, 2020 |
| Contact name |
Jun Wasaki |
| E-mail(s) |
junw@hiroshima-u.ac.jp
|
| Organization name |
Hiroshima University
|
| Department |
Graduate School of Biosphere Science
|
| Street address |
Kagamiyama 1-7-1
|
| City |
Higashi-Hiroshima |
| State/province |
Hiroshima |
| ZIP/Postal code |
739-8521 |
| Country |
Japan |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE10335 |
Transcriptional profiling of shoot of OsPI1 knockdown rice |
|
