|
| Status |
Public on Jun 26, 2008 |
| Title |
Wild-type_thymus_M1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from several cell lines
|
| Organism |
Mus musculus |
| Characteristics |
Wild-type
|
| Treatment protocol |
4-week-old mice of both sexes were exposed to four weekly doses of 1.75 Gy/dose of ionizing gamma
|
| Growth protocol |
Tumors develop in vivo after irradiation of animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by combination of Trizol reagent (Invitrogen) and purification using the RNeasy Protect kit (Qiagen, Hilden). The quality of the RNA was evaluated using the BioAnalyzer system (Agilent).
|
| Label |
Cy3
|
| Label protocol |
Twenty-five micrograms of the test or reference amplified RNAs were labeled with fluorescent Cy5 and Cy3 (Amhersam, Sunnyvale), respectively using the SuperScript II RNase H Reverse Transcriptase KIT (Invitrogen, USA).
|
| |
|
| Channel 2 |
| Source name |
Normal Thymus M1
|
| Organism |
Mus musculus |
| Characteristics |
Wild-type
|
| Treatment protocol |
4-week-old mice of both sexes were exposed to four weekly doses of 1.75 Gy/dose of ionizing gamma
|
| Growth protocol |
Tumors develop in vivo after irradiation of animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by combination of Trizol reagent (Invitrogen) and purification using the RNeasy Protect kit (Qiagen, Hilden). The quality of the RNA was evaluated using the BioAnalyzer system (Agilent).
|
| Label |
Cy5
|
| Label protocol |
Twenty-five micrograms of the test or reference amplified RNAs were labeled with fluorescent Cy5 and Cy3 (Amhersam, Sunnyvale), respectively using the SuperScript II RNase H Reverse Transcriptase KIT (Invitrogen, USA).
|
| |
|
| |
| Hybridization protocol |
Hybridizations were performed at 55°C for 17 h using the SlideHyb Microarray Hybridization buffer (Ambion).
|
| Scan protocol |
the slides were scanned in an Agilent G2565BA scanner.
|
| Description |
Normal thymus-control
|
| Data processing |
Images were then analyzed with GenePix 5.1 (Axon Instruments, Inc., Union City, CA) and the TM4 software
|
| |
|
| Submission date |
Mar 17, 2008 |
| Last update date |
Jun 26, 2008 |
| Contact name |
Marcos Malumbres |
| E-mail(s) |
mmm@cnio.es
|
| Organization name |
Centro Nacional de Investigaciones Oncológicas (CNIO)
|
| Department |
Molecular Oncology
|
| Lab |
Cell Division and Cancer
|
| Street address |
Melchor Fernandez Almagro 3
|
| City |
Madrid |
| ZIP/Postal code |
E-28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6593 |
| Series (2) |
| GSE10859 |
Expression patterns of cDNAs in gamma-irradiation induced T-cell lymphomas |
| GSE10861 |
T-cell lymphoblastic lymphomas induced by gamma-irradiation |
|
